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(amino acids 1–23, serving as a positive control) were individually fused in-frame to yeast invertase cloned in the pSUC2 vector. When transformed with either construct, the invertase mutant yeast strain YTK12 was able to grow on the YPRAA medium and convert 2, 3, 5-triphenyltetrazolium ...
Briefly, a rice RCK RNAi vector (p35S RCK-RNAi) was constructed with a 514-bp cDNA fragment of RCK (nt 2 409-2 922), which shares no similarity to other sequences in the rice genome. After transformation, 40 independent T0 transformants were obtained and most of them were confirmed to...
The PCR-amplified DNA was cloned into vector pEASY-T1 Simple Cloning Vector (TransGen Biotech, China) and transformed into DH5α cells (TransGen Biotech, China), sequenced by terminal labeling using BigDye Terminator v3.1 (Applied Biosystems, http://www.appliedbiosystems.com/). The sequenc- ...
Hence, we tested the repressive ability of the SRDX motif in the rGAL4 vector. SRDX repressed rGAL4 activation of reporter genes up to 30%, strengthening our claim that yeast rGAL4-repressor motif fusion can be effectively used for tran-srepression assays. Interestingly, DLN144 proved to ...
In determining the pure rice pixels, they used support vector machine classifier. In addition, they used "rice area fraction index" in identifying the mixed pixels (i.e., mix of rice with other crops). Employed NDVI images, i.e., one image during the peak greenness stage in 1989; and ...