For example, RiboTag-expressing transgenic mice were recently used to compare differential gene expression responses to cocaine in striatal neurons expressing D1 and D2 dopamine receptors (Chandra et al, 2015). Because RiboTag and BacTRAP allow the measurement of ribosome-associated mRNA, it is not...
Characterization of microglial transcriptomes in the brain and spinal cord of mice in early and late experimental autoimmune encephalomyelitis using a RiboTag strategy Shaona Acharjee, Paul M. K. Gordon, Benjamin H. Lee, Justin Read, Matthew L. Workentine, Keith A. Sharkey & Quentin J...
Here we describe our adaptation of a ribosomal capture strategy that was designed to be used in transgenic mice expressing tagged ribosomal subunits (RiboTag) in specific cell types, thereby allowing measurement of translating RNAs from desired cell types within complex tissues. Using this strategy we...
Conversely, our data highlight the added value of RiboTag profiling for assessing the lineage accuracy of Cre recombinase expression in transgenic mice. Collectively, this study indicates method-based biases, reveals observer effects and establishes RiboTag-based translatome profiling as a valuable ...
In mice subjected to middle cerebral artery occlusion and reperfusion and contralateral controls, we sequenced ribosome-bound RNAs from perivascular astrocyte endfeet and identified 205 genes that were differentially expressed in the endfoot translatome after ischemia. The main biological processes ...