This method provides a snapshot of all ribosomes active in a cell at a specific time; it allows detailed in vivo analysis of protein production.
利用Ribo-seq,研究者能从基因组水平检测蛋白质的翻译状况,获得全面的、高质量的蛋白质翻译速度情况,了解蛋白质表达情况及其丰度;还能直接对翻译过程进行研究。
CD genomics provides a ribosome-profiling strategy that is based on the deep sequencing of ribosome-protected mRNA fragments and enables genome-wide investigation of translation with sub cordon resolution. Using nuclease digestion, the ribosome position and the translated message can be precisely determine...
翻译组测序Ribosome Profiling(或称Ribo-seq)是针对与核糖体结合,长度约为30nt,正在翻译的mRNA片段进行富集、测序和定量(图1)[2-4]。据此获取每种转录本上核糖体的分布,并可推测起始密码子位置(包括非ATG起始)以及uORFs,并可进一步分析翻译效率,解析翻译调控机制。 核糖体图谱分析(Ribosome profiling),是翻译调控主...
Our Ribo-sequencing bioinformatics service provides rich annotations, comprehensive data analyses, and publication-ready graphics. We also provide Integrative Analysis of Ribo-seq and RNA-seq, which combines transcriptional and translational profiling data to gain comprehensive insights of gene expression. ...
Ribosome-profiling techniques were developed in response to the importance of measuring gene expression at the translational level. CD Genomics offers comprehensive Ribo-Seq services.
Ribo-seq可以找到很多公司做，但是想做一个polysome profiling的图，在不同处理下，能看出来大小亚基和...
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Ribosome profiling (also called Ribo-Seq) is a relatively new method for studying translational regulation of biological processes. Based on the assumption that ribosomes bound to mRNA molecules are all actively decoding the nucleotide sequence to produce polypeptides, ribosome profiling uses next-...
Ribosome profiling or ribo-seq is a new technique that provides genome-wide information on protein synthesis (GWIPS) in vivo. It is based on the deep sequencing of ribosome protected mRNA fragments allowing the measurement of ribosome density along all RNA molecules present in the cell. At the ...