ribosomal RNA depletion kits for RNA-Seq, ribosome profiling, degraded RNA. Kits for single and multiple species including human, mouse, bacteria, etc.
Figure 1. Methods for selecting RNA species of interest. (A)Total RNA content in eukaryote cell,(B)selection of mRNA content in a eukaryote cell by oligo-dT binding usingDynabeads mRNA DIRECT Micro kit, and(C)complete transcriptome isolation with theRibominus Eukaryote ...
Results We find that all of the kits are capable of performing significant ribosomal depletion, though there are differences in their ease of use. All kits were able to remove ribosomal RNA to below 20% with intact RNA and identify ~14,000 protein coding genes from the Universal Human ...
5. Use the RNA Clean & Concentrator-5 kit (Zymo research) to concentrate the ribodepleted samples, to carry out size selection and to digest any remaining DNA using DNase I treatment as de- scribed [27] Ribosomal RNA depletion in planarian species related to S. mediterranea Ribosomal DNA ...
Genome BiologyCiulla, D., Giannoukos, G., Earl, A., Feldgarden, M., Gevers, D., Levin, J., et al. (2010). Evaluation of bacterial ribosomal RNA (rRNA) depletion methods for sequencing microbial commu- nity transcriptomes. Genome Biology, 11(Suppl 1), P9....
Research2MeVt0oaa0lrul7y.mgoeld8, Issue 10, Article R216 Open Access The ribosomal protein genes and Minute loci of Drosophila melanogaster Steven J Marygold*, John Roote†, Gunter Reuter‡, Andrew Lambertsson§, Michael Ashburner†, Gillian H Millburn†, Paul M Harrison¶, Zhan ...
For RNA-Seq library preparation, 0.5 µg of total RNA (with a TruSeq Stranded mRNA Library Prep Kit; Illumina) or 5 ng of total RNA (with a SMARTer Stranded Total RNA-Seq Kit v3; Takara Bio) was used. For the quantification of mRNAs in the ribosome-free fraction and ribosome...
One mg RNA in 20 ml water was mixed with 42.5 ml bisulfite solution (Epitect Kit, Qiagen) and 17.5 ml DNA protect buffer (Epitect Kit, Qiagen) in a thin wall 100 ml PCR tube. In a thermo cycler, the following programme was run overnight: 70 °C for 10 min and 60 °C for 60...
To verify this point, we depleted Fbxo22 with shRNA to stabilize KDM4B. Fbxo22 depletion increased the steady-state level of KDM4B-S666A (Additional File 1: Fig. S5A) but did not allow KDM4B-S666A to accumulate at DSB sites (Additional File 1: Fig. S5B), indicating that KDM4B ...
Reverse transcription of 200 µg RNA was performed using a kit containing SuperScript III reverse transcriptase (ThermoFisher, 18,080,093) in the presence of random hexamers. The resulting cDNA was diluted 1:4, and 1 µL of the dilution was used in a qRT-PCR reac- tion mix ...