Human RNase P has been purified more than 2000-fold from HeLa cells. In addition to the RNA component, H1 RNA, polypeptides of molecular masses 14,20,25,30,38, and 40 kDa copurify with the enzyme activity. Sera from two different patients with the autoimmune disease scleroderma were used ...
The RNA component of the enzyme was found to be structurally and evolutionally related to RNase P RNA, the ribonucleoprotein endoribonuclease that processes the 5′ end of tRNA, and several protein components are shared between the two enzyme complexes. The data suggest that the enzymes, while ...
*# Baer, et al., "Structure and Transcription of a Human Gene for H1 RNA the RNA Component of Human RNase," Nucleic Acids Res., 18:97-103 (1989). *# Bartkiewicz, et al., "Identification and characterization of an RNA molecule that copurifies with RNase P activity from HeLa cells...
suggests some likely functions of P3 in stabilizing the enzyme's structure and in interactions with pre-tRNA [90]. In human nuclear RNase P, Rpp21 binds to H1 RNA and also to the P3 domain [91]. InS. cerevisiaeRNase MRP and RNase P, Pop6 and Pop7 form a heterodimer that binds direc...
Human ribonuclease P - Jarrous, Altman - 2001 () Citation Context ...r tRNAs. In Bacteria and some Archaea, the RNA component alone exhibits catalytic activity in vitro; however, the protein subunit C5 is essential for activity in vivo and is encoded by the rnpA gene (=-=Altman & ...
The hybrid binding domain (HBD) is a conserved fold present in ribonucleases H1 that selectively recognizes RNA-DNA hybrids, which are structures present in cellular R-loops and participate in diverse biological processes. We engineered multivalent HBD proteins to create high-affinity hybrid binders...
RNaseMRP has been isolated from various organisms including human, mouse, rat, cow,Xenopus,yeast,Arabidopsis,and tobacco (see Reilly and Schmitt5for review). In yeast,Saccharomyces cerevisiae,the enzyme complex is known to consist of an RNA component of 340 nucleotides in length and nine protein...
tsRNA expression levels in the sperm, and angiogenin (ANG) in the mouse caput epididymis regulates sperm tsRNAs and mediates paternal inflammation-induced metabolic disorders in the offspring [15]. RNH1, also named ribonuclease inhibitor 1, is generally expressed in human tissues [16]. This protei...
HIV RT is multifunctional, with both RNA-dependent and DNA-dependent DNA polymerase activity, as well as ribonuclease H (RNase H) activity that degrades the RNA component of the RNA/DNA hybrids duplex intermediate formed during reverse transcription. All of these RT activities are essential for ...