Determine the level of fluorophore incorporation (the F/P ratio) by measuring the absorbance of the labeled protein at 520 nm and 280 nm. Labeled proteins having a 520 nm/280 nm ratio of absorbency of 0.3 to 0.8 should perform well in most applications (Titus et al., 1982). At the tim...
The amino isologs offluorescein, namely, therhodamines, were first described in the 1880s.75–77The simplestrhodamine,rhodamine 110(49;Fig. 1.5), has similaroptical propertiesto fluorescein (λmax/λem=496/517nm,ɛ=7.4×104M−1cm−1, andΦ=0.92) but, likeaminocoumarins, is pH insensi...
The absorbance and emission maxima of Carboxyrhodamine 110 DBCO conjugates are 501 nm and 523 nm, respectively. Chemoselective Ligation via Copper-free Click Chemistry Catalog NumberProductDataSheetSizeAVAILABILITYPriceQty FCC052 DBCO-Carboxyrhodamine 110, 1 mg Datasheet 1mg In stock $131.00 ...
Molecular weight 380.82 Solvent DMSO Spectral properties Absorbance (nm) 497 Extinction coefficient (cm-1M-1) 85200 Excitation (nm) 508 Emission (nm) 528 Documents Certificate of origin Safety data sheet Product protocol Certificate of analysis
Tetramethylrhodamine-5-maleimide is widely used for thiol modifications of peptides and proteins. Specifications Chemistry CAS registry number 174568-67-3 Molecular Formula C28H23N3O5 Molecular Mass/ Weight 481.5 Properties Absorbance (nm) 540 Emission (nm) 567 Color Orange Quantity & ...
Moreover, both nanoheterostructures showed strong levels of visible light absorbance relative to UV absorber pristine CeO2 NPs. Moreover, Mott-Schottky measurements were collected to determine electrochemical flat potential and band gap structures, and based on the derived results, a schematic diagram ...
First, the absorbance values of the rhodamine dyes were measured as a function of the dielectric constant of the solvent to determine the D50 value (dielectric constants at half-maximum absorbance), a characteristic parameter reporting on the positions of the open-closed equilibrium (Fig. 1d)31–...
and the supernatant was taken after centrifugation for 5 min. Using deionized water as a reference, after dilution (λmax= 554 nm) and determination of the absorbance value according to the standard curve to calculate the residual RhB wastewater mass concentration, the RhB wastewater adsorption rate...
The degradation of RhB was monitored by measuring the 555 nm absorbance peak intensities of the RhB solution using a UV-vis spectrophotometer (LABOMED UV-2602). The concentration of RhB in the solution was determined according to the Lambert-Beer law. The relationship between reaction concentrations...
The activity of the Fe2P-iron phosphate thin film as an anode material for organic molecule degradation was studied by monitoring its effect on the absorbance spectra of a solution of RhB dye. As shown in Figure 4a, the 550 nm absorption peak of RhB was completely degraded to baseline in ...