RT-PCR: rhinovirus, enterovirus, and coronavirus median age: 7.2 years ii) specific symptoms/symptom frequency suggestive of asthma Prospective birth cohort At least one parent with skin test confirmed respiratory allergy viral culture: RSV, influenza A and B, parainfluenza, rhinovirus, enteroviruses...
RT-PCR assays are more rapid and sensitive than isolation techniques for the detection of HRV RNA in clinical specimens. Historically, HRV infections have been detected by virus isolation in cell culture followed by acid lability testing to distinguish them from enterovirus infections. Virus isolation...
in the genusEnterovirus. HRV-C isolates do not grow in standard cell culture (e.g.HeLaor embryonic lung fibroblasts), and this distinguishing feature most likely has postponed their discovery until recently[8–12]using molecular diagnostics that do not require virus isolation or passaging. Sequenci...