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Detection of HRV was performed via reverse transcription on RNA extracts using RevertAid H Minus First Strand cDNA synthesis kit (Fermentase, USA). PCR was performed for all the samples using primers that amplified a fragment encompassing the VP4/VP2 region and the hyper-variable region in the...
1, Masande Yalo 2, Rajan Sharma 2,†, Masixole Makhaba 2, Ahmed A. Hussein 2and Wilfred T. Mabusela 1,* 1 Department of Chemistry, University of the Western Cape, Private Bag X17, Bellville 7535, South Africa 2 Chemistry Department, Cape Peninsula University of Technology, Symphony Rd...
Column chromatography (CC) was performed using normal-phase silica gel, while thin layer chromatography (TLC) was performed on silica gel aluminum sheets (Silica gel 60 F254, Merck, Rahway, NJ, USA). Visualization of the spots on TLC sheets was achieved using vanillin sulfuric acid reagent ...
Column chromatography (CC) was performed using normal-phase silica gel, while thin layer chromatography (TLC) was performed on silica gel aluminum sheets (Silica gel 60 F254, Merck, Rahway, NJ, USA). Visualization of the spots on TLC sheets was achieved using vanillin sulfuric acid reagent ...