进一步机制研究表明,RfxCas13d的旁切活性能够将28s rRNA切割成两段,造成细胞翻译功能受损和ZAKα-JNK/p38-IEG(immediate early gene)通路的活化。 总而言之,这项研究为RfxCas13d在哺乳动物细胞中的旁切活性提供了新的机制见解,并警示...
(A)信号终止子,pVS1 RepA,pVS1复制起点,CaMV 35S启动子,RfxCas13d基因,NOS终止子;其中,RfxCas13d基因序列如SEQ ID No.1所示,NOS终止子序列如SEQ ID No.2所示.本发明利用带有GFP荧光标签的芜菁花叶病毒(TuMVGFP)侵染性克隆来指示RNA病毒自然侵害植物,利用RfxCas13d蛋白和sgRNA结合可以定向特定位置切割RNA.本...
相较于传统的RNA干扰方法(包括shRNA和siRNA),创新型的CRISPR-RfxCas13d/BSJ-gRNA系统能够高效地敲低环形RNA表达且不影响其亲本线形mRNA表达,呈现出了更好的环形RNA敲除效果和靶向效应。在此基础上,研究人员成功构建并利用多个RfxCas13d/BSJ-gRNA文库针对一系列人源细胞系中高表达的环形RNA开展功能靶向筛选 。利用...
PMLAAV-ENCMV-RFXCAS13D CBNumber: CB213304494 分子式: 分子量: 0 MOL File: Mol file 化学性质安全信息用途供应商1 PMLAAV-ENCMV-RFXCAS13D化学性质 安全信息 PMLAAV-ENCMV-RFXCAS13D性质、用途与生产工艺 PMLAAV-ENCMV-RFXCAS13D上下游产品信息 ...
pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE质粒 货号:#138148 货号产品名称规格库存价格数量购买 #138148pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE质粒EA干粉 立即咨询 +- 产品介绍 相关产品 ...
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Here, we describe a detailed protocol based on this RfxCas13d/BSJ-gRNA system for large-scale functional circRNA screening in human cell lines. The protocol includes gRNA library design, construction and transduction, analysis of screening results and validation of functional circRNA candidates. In ...
Here we show that RfxCas13d, also known as CasRx, a widely used Cas13 system, can cause collateral transcriptome destruction when targeting abundant reporter RNA and endogenous RNAs, resulting in proliferation defect in target cells. While these results call for caution of using RfxCas13d for ...
RfxCas13dBackground:The CRISPR-Cas13 system is an RNA-guided RNA-targeting system and has been widely used in transcriptome engineering with potentially important clinical applications. However, it is still controversial whether Cas13 exhibits collateral activity in mammalian cells. Results:Here, we ...
一种蛋白序列MINI RFXCAS13D,属于生物工程技术领域.本发明的目的是提供一种编辑活性高,CAS13D蛋白小的氨基酸序列小的蛋白序列MINI RFXCAS13D.本发明氨基酸序列小的蛋白序列MINI RFXCAS13D具有SEQ ID NO:1所示的氨基酸序列,MINI RFXCAS13D蛋白是RFXCAS13D的人工缺失版本.本发明能精确靶向RNA序列,并产生切割,使...