FACS analysis of RFP-positive cells in stable HEK-293 cells.Sheng, WenHongmei, ZhangYasha, LiNing, WangWenwen, ZhangKe, YangNingning, WuXian, ChenFang, DengZhan, Liao
'In estrogen receptor-positive (ER+) breast cancer cells, BCL2 overexpression contributes to antiestrogen resistance. Direct targeting of the antiapoptotic BCL2 members with GX15-070 (obatoclax), a BH3-mimetic currently in clinical development, is an attractive strategy to overcome antiestrogen res...
Functional & Integrative Genomics (2023) 23:42 https://doi.org/10.1007/s10142-023-00960-1 METHODOLOGY CRISPR/Cas9‑mediated knockin of IRES‑tdTomato at Ins2 locus reveals no RFP‑positive cells in mouse islets Xueling Zhou1,2 · Qi Fu1 · Tao Yang1 · Min...
(RFP), with the transduction efficiency of BacMam technology. Transduction or even co-transduction can be performed in a wide variety of cells, including neurons and neural stem cells (NSCs) using a simple protocol that involves adding the BacMam 2.0 chimeric reagent to cells, incubating over...
22、-RFP plasmid (positive contrast); Lane 3: total protein samples in bacterium fluid containing un-loaded pET-28-RFP plasmid (negative contrast).(1)用IPTG诱导表达后试管内菌液呈现红色,说明含外源基因RFP的质粒在大肠杆菌体内成功表达。(2)由图2可以看出,阳性对照比阴性对照多了一条带,即为表达的目...
Immunostaining of HeLa cells, transiently transfected with different fluorescent proteins. Primary antibody: pan-RFP (pabr1) 1:800 for 1 h RT. Secondary: goat anti-rabbit. Upper row shows the innate signals from transfected fluorescent proteins, nuclei are in cyan. Lower row shows the signals fr...
Try varying particle-to-cell ratio (PPC), incubation volume, temperature and, cell density (if adherent cells are transduced). For adherent cells, we recommend a confluence of about 70%. Following the PPC, adjusting the volume is the next best parameter to change to optimize protein expression...
Immunostaining of HeLa cells, transiently transfected with different fluorescent proteins. Primary antibody: pan-RFP (pabr1) 1:800 for 1 h RT. Secondary: goat anti-rabbit. Upper row shows the innate signals from transfected fluorescent proteins, nuclei are in cyan. Lower row shows the signals fr...
PTEN truncation mutants or empty vector were coexpressed with RFP in 293 cells (Figure 1D), and immunoprecipitation of transfected cell lysates with M2 beads revealed that RFP did not interact with the truncation mutant that lacks the Tail domain (NC2) (lane 2), whereas the PDZ-binding ...
Positive IF/ICC detected inA549 cells Recommended dilution ApplicationDilution Immunohistochemistry (IHC)IHC : 1:50-1:500 Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800 It is recommended that this reagent should be titrated in each testing system to obtain optimal results. ...