excess of nanobody35 (Nb35; see below for protocols of expression and purification) was added at the beginning of this incubation process to improve complex stability. The resin was collected by centrifugation at 800gfor 5 min and washed with 4 column volumes of 20 mM HEPES, pH 7.5, ...
The Ti disk substrate was placed at a distance of 50 mm above the TiO2 target and kept at 600 ˝C during the deposition. Four samples were prepared at different deposition times (1, 3, 6 and 8 h). A thin platinum coating (ca. 20 mg¨ cm´2 loading) was deposited on the ...