Since the epitopes of the vaccine model were compatible with both the human and mouse immune systems, both eukaryotic and prokaryotic expression vectors were generated. For constructing the adenovirus-based vaccine, the Kozak sequence containing a start codon that controls the translation initiation was ...
The physical and chemical properties of vaccine such as theoretical pH, half-life, molecular weight, grand average of hydropathicity, aliphatic and instability indexes, were computationally analyzed using the Expasy tool of Protparam18. Furthermore, the designed multi-epitope vaccine was subjected to ...
Since the epitopes of the vaccine model were compatible with both the human and mouse immune systems, both eukaryotic and prokaryotic expression vectors were generated. For constructing the adenovirus-based vaccine, the Kozak sequence containing a start codon that controls the translation initiation was ...
(20) IEDB population coverage server: http://tools.iedb.org/population/ (21) MHCcluster 2.0 tool: https://servi ces.healthtech.dtu.dk/services/MHCcluster-2.0/ (22) ExPASy ProtParam tool: https://web.expasy.org/...
The 3D building models were performed using Swissmodel server (https://swissmodel.expasy.org/), which allows homology modeling and the creation of 5 final models. At the same time, we also submit the primary sequence to the Robetta server. The Robetta server (https://robetta.bakerlab.org/...
Gasteiger E et al (2005) Protein identification and analysis tools on the ExPASy server. The proteomics protocols handbook, pp 571–607 Gill SC, von Hippel PH (1989) Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem 182(2):319–326. https://doi.org/...
Since the DV-1 protein had 354 amino acids and HV-1 had 335 amino acids, therefore, after reverse translation, the number nucleotides of the probable DNA sequence of DV-1 would be 1062 nucleotides long and HV-1 would be 1005 nucleotides long. The codon adaptation index (CAI) value of 0....
Expasy Protparam (https://web.expasy.org/protparam/) was used to predict various physicochemical properties like theoretical isoelectric point (pI), amino acid composition, in vitro and in vivo half-life, instability and aliphatic index, molecular weight (MW), and grand average of hydropathicity (...
critical component in the development of mRNA vaccines, as it directly impacts the translation efficiency and expression of the vaccine construct. However, among the nine articles, only Bhattacharya et al. [29] and Khan et al. [31] delve into this aspect, employing different tools and ...