逆转录聚合酶链反应（RT-PCR）是一种实验室技术，能够将RNA逆转录为DNA（互补DNA或cDNA），并利用聚合酶链反应（PCR）扩增特定的DNA目标。此技术主要用于测量特定RNA的量。实时PCR或定量PCR（qPCR）通过荧光监测扩增反应来实现，联合RT-PCR和qPCR通常用于基因表达分析，在研究和临床环境中对病毒RNA进行定量...
Reverse transcription coupled to the polymerase chain reaction (RT-PCR) is commonly used to detect the presence of mRNAs, pre-mRNAs, or other types of RNA such as noncoding RNAs. The method involves using a primer annealed to the RNA of interest. For mRNA, the primer is usually a ...
逆转录聚合酶链式反应( RT-PCR ) Reverse transcription polymerase chain reaction https://en.wikipedia.org/wiki/Reverse_transcription_polymerase_chain_reaction 历史 自1977 年推出以来,Northern 印迹已被广泛用于 RNA 定量,尽管它存在以下缺点:(a) 耗时的技术,(b) 需要大量 RNA 进行检测,以及 (c) 在低丰度...
Reverse transcription polymerase chain reaction内分泌关键词: 内分泌 实验技术 实验技术 词汇介绍 拓展阅读解析 Reverse 英/rɪ'vɜːs/ 美/rɪ'vɝs/ 释 义 v. 颠倒;撤销;反转;交换;放弃立场;倒车;打对方付费的电话;(使铅字、图案)印成白或浅色 n. 逆向;相反;背面;倒档;失败;(美橄)横式传球...
Reverse transcription-polymerase chain reaction (RT-PCR) is a sensitive in vitro method and has a crucial role in medical science andbiomaterial fields. RT-PCR is used for detecting and comparing the levels of mRNA and the surface proteins (Leong et al., 2007; Wang and Brown, 1999...
Reverse transcription-polymerase chain reaction (RT-PCR)|逆转录-聚合酶链反应(RT-PCR) 基本信息 更多信息 中文名称: 逆转录-聚合酶链反应(RT-PCR) 中文同义词: 逆转录-聚合酶链反应(RT-PCR) 英文名称: Reverse transcription-polymerase chain reaction (RT-PCR) 英文同义词: Reverse transcription-polymerase ...
Expression of the TFPI-2 gene was determined by Reverse transcription-polymerase chain reaction(RT-PCR) and Western blotting. 方法:将重组质粒pEGFP-C1-TFPI-2通过脂质体介导转染胰腺癌细胞系Panc-1细胞,G418筛选获得阳性细胞克隆后,用逆转录-聚合酶链反应(RT-PCR)和免疫印迹(Western blot)技术分别检测转染...
DNA-dependent DNA polymerase activity (if present) recognizes the single-stranded cDNA as a template, uses an RNA fragment as a primer, and synthesizes the second-strand cDNA Double-stranded cDNA is formed Learn more: Reverse transcription reacti...
Reverse transcription-quantitative polymerase chain reaction: description of a RIN-based algorithm for accurate data normalization Background Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is the gold standard technique for mRNA quantification, but approp... Alexandre,Ho-Pun-Cheung,Ca...
Reverse transcription–polymerase chain reaction–based methodology to quantify differential gene expression directly from microdissected regions of frozen... (1997) Reverse transcription-polymerase chain reaction-based methodology to quantify differen- tial gene expression directly from microdissected regions of...