During reverse transcription of the genomic RNA of human immunodefi ciency virus type 1 (HIV-1) into double-stranded DNA, reverse transcriptase (RT) must accommodate RNA – RNA, DNA – RNA, RNA – DNA and DNA – DNA hybrids as primer-template. In this study, we examined extension of ...
Thermo Scientific reverse transcriptases selection guide Reverse transcriptases are selected for their sensitivity and thermostability. The attributes of various Thermo Scientific reverse transcriptases are listed inTable 1. Table 1. Attributes of Thermo Scienti...
Choose from a variety of reverse transcriptase stand-alone enzymes, master mixes, cDNA synthesis kits, primers, and dNTP solutions to suit your application. Invitrogen SuperScript IV products aid in exceptional cDNA synthesis from any type of template RNA. Use the selection tool to find the right...
Reverse Transcriptase: M-MLV Reverse Transcriptase Ribonuclease H Activity: Reduced Format: Tube(s) Template: ssDNA, ssRNA, RNA-DNA Hybrid Sensitivity: Low Product Size: 40,000 units Concentration: 200 U?μl Enzyme Function: RNA dependent DNA Polymerase Shipping Condition: Wet Ice Number of Reacti...
A 30-nucleotide DNA aptamer (5'-AGGAAGGCTTTAGGTCTGAGATCTCGGAAT-3', denoted PF1) selected for high affinity to human immunodeficiency virus reverse transcriptase (HIV RT) using a primer-free SELEX (systematic evolution of ligands by exponential enrichment) method was characterized to determine feat...
An additional mutation in reverse transcriptase (RT), G490E, was found to facilitate the forced use of tRNALys1,2 as the primer. In the current study, we have investigated the impact of the G490E mutation in RT on the selection and use of alternative primers by HIV-1. Viruses were ...
M-MLV Ⅱ Reverse Transcriptase(200U/ul) 价格￥904.8 起批量≥1盒 最小起订1盒 供货总量29盒 发货地址上海市 建议售价￥904.8/盒 更新日期2025年01月25日 产品规格10000U 即时洽谈立即询价查看联系方式 收藏产品发送留言 VIP6年 上海博湖生物科技有限公司 ...
Previous results using a SELEX (Systematic Evolution of Ligands by Exponential Enrichment)-based approach that selected DNA primer-template duplexes binding with high affinity to HIV reverse transcriptase (RT) showed that primers mimicking the 3′ end, and in particular the six nt terminal G tract...
1) Reverse transcriptase-nested primers-polymerase chain reaction 反转录套式聚合酶链反应2) multiplex reverse transcription-nested polymerase chain reaction 复合反转录-套式聚合酶链式反应 1. A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type ...
Selection of high-affinity RNA ligands to reverse transcriptase: Inhibition of cDNA synthesis... Chen, H. & Gold, L. 1994 Selection of high-affinity RNA ligands to reverse transcriptase: inhibition of cDNA synthesis and Rnase H activity. ... Chen,Hang,Gold,... - 《Biochemistry》 被引量: ...