RPPApipe: a pipeline for the analysis of reverse-phase protein array data. Biosystems. 2014;122:19–24.Eichner J, Heubach Y, Ruff M, Kohlhof H, Strobl S, Mayer B, et al. RPPApipe: a pipeline for the analysis of reverse-phase protein array data. Biosystems 2014;122:19e24.
Reverse Phase Protein Array(RPPA) 蛋白组学技术在外泌体分析中的应用 外泌体(Exosome)是一种能被大多数细胞分泌的微小膜泡,也是细胞外囊泡(EV)的一种主要类型,直径大约40-160 nm,具有脂质双层膜结构,能很好的保护其包被的物质,内含大量的核酸,包括DNA、RNA以及蛋白质和其它小分子代谢产物。在最近的癌症研究中...
Reverse Phase Protein Array (RPPA) is an antibody-based high throughput proteomic approach that enables the simultaneous quantification of large sample sizes (up to 1500 samples at once). Examples of RPPA use include biomarker quantification in clinical samples, mapping signaling interactions through ...
Reverse-phase protein array (RPPA) technology uses panels of high-specificity antibodies to measure proteins and protein post-translational modifications in cells and tissues. The approach offers sensitive and precise quantification of large numbers of samples and has thus found applications in the analys...
Reverse phase protein arrays (RPPA) have been demonstrated to be a useful experimental platform for quantitative protein profiling in a high-throughput format. Target protein detection relies on the readout obtained from a single detection antibody. For this reason, antibody specificity is a key fact...
Reverse-Phase Protein Array Super Position and Concentration Evaluation The RPPASPACE R package provides tools for the analysis of reverse-phase protein arrays (RPPAs), which are also known as 'tissue lysate arrays' or simply 'lysate arrays'. The package's primary purpose is to input a set of...
Reverse Phase Protein Array (RPPA) of High Risk ALL In a NOD/SCID/huALL model we have recently shown that short time to leukemia (TTLshort) of xenografted patient samples determines poor patient outcome. In ... F Seyfried,B Accordi,M Queudeville,... - 《Klinische Pädiatrie》 被引量...
However, this method leads to a large amount of data points to analyze, requires substantial cell amounts, and is labor-intensive when many compounds or parameters have to be tested. In this context, we propose to couple reverse-phase protein array (RPPA) technology to CETSA (Fig. 1B). ...
Reverse-phase protein array (RPPA) technology uses panels of high-specificity antibodies to measure proteins and protein post-translational modifications in cells and tissues. The approach offers sensitive and precise quantification of large numbers of samples and has thus found applications in the ...
Reverse phase protein arrays (RPPA) are an efficient, high-throughput, cost-effective method for the quantification of specific proteins in complex biological samples. The quality of RPPA data may be affected by various sources of error. One of these, spatial variation, is caused by ...