Conspecific aggressiveness often increases after social isolation for species that are not entirely solitary, and this increased aggression could also be reversed after resocialization. However, literature on this aggression plasticity refers to either permanently social or low-level subsocial species in in...
For cellular protein isolation, cells were rinsed with PBS (pH 7.4) and subsequently lysed in RIPA lysis buffer supplemented with PMSF (1:100, Beyotime Biotechnology) and a phosphatase inhibitor cocktail (1:100, Thermo Fisher Scientific) at 4 °C for 30 min. For tissue protein isolation,...
Interoperability is one of the main reasons to reverse engineer APIs. Unlike a few decades ago, when software could exist in isolation, nowadays, a program runs on a complicated operating system and involves communication with several libraries created by different people. Reverse engineering APIs for...
Nursing educationThe paper reports on an investigation of the characteristics associated with small and medium-sized enterprise (SME) exporters that operate in international healthcare markets. A framework is initially developed to analyse (a) the factors that stimulate such firms to venture overseas, ...
Polydispersity can occur due to the size distribution in a sample, or agglomeration or aggregation of the sample, during isolation or analysis. PdI values range from 0.0 (for a perfectly uniform sample with respect to the particle size) to 1.0 (for a highly polydisperse sample with multiple ...
While it can treat various water impurities effectively, NF in isolation falls short in adequately reducing the high salinity inherent in seawater to produce potable water. This shortcoming is where the combined might of NF and RO shines through, achieving better desalination results [11]. Emerging...
Single clone isolation from the HEK293TACE2, A549ACE2, and NCI-H23ACE2 transduced cell pools was carried out using the array dilution method in 96-well plates [12]. Cell colonies from single clones were collected 2–3 weeks after seeding and were expanded in increasingly larger cell ...
2.7. RNA Isolation and RT-qPCR Analysis Total RNA was extracted by using TRIsure Reagent (Bioline, London, UK), as instructed by the manufacturer. A260/A280ratio in a NanoDrop ND-1000 Spectrophotometer (Thermo Scientific) was used to determinate RNA quality. Momentarily, RNA (1 µg) was ...
Isolation of a novel cutinase homolog with polyethylene terephthalate-degrading activity from leaf-branch compost by using a metagenomic approach. Appl. Environ. Microbiol. 2012, 78, 1556–1562. [Google Scholar] [CrossRef] [PubMed] [Green Version] Zhu, B.; Wang, D.; Wei, N. Enzyme ...