Retro Biosciences has 1 investors. Sam Altman invested in Retro Biosciences's Angel funding round. First funding Last Funding Investor Rounds Board Seats Type Location 4/5/2022 4/5/2022 Sam Altman 1 Angel Angel Investor (Individual) California Learn more by requesting a demo Trusted by the worl...
Long-read, whole-genome sequence analysis Sequenced reads were mapped to the human reference genome (GRCh37) using pbmm2 (https://github.com/PacificBiosciences/pbmm2), a wrapper for minimap2 (ref.71). Sequences for L1-supporting reads near source elements were extracted and mapped to the L1...
All antibodies were obtained from BD Biosciences (San Jose, CA, USA). Single cell suspensions were prepared from spleen and BM for cell sorting. Humanized NSG animal from all four groups were analysed from pooled cells and tissues. Human CD45+ cells obtained by magnetic beads were incubated ...
The cells were collected on day 7–8 post-transfection and the resultant EGFP positive cells were analyzed using BD Accuri C6 Plus Software v.1.0.23.1 (BD Biosciences). The FITC channel was used to count the EGFP positive cells out of 30,000 cells. The number of the EGFP-positive cells ...
Then, hESCs were plated on culture flasks coated with 1.2% Matrigel (BD Biosciences, San Jose, CA), followed by incubation in a humidified incubator of 5% CO2 at 37 °C. Then, hESEVs were isolated and maintained with culture medium being refreshed every 24 h. hESCs were passaged ...
The DNA obtained was amplified by quantitative PCR (qPCR) using PerfeCTa SYBR Green SuperMix for iQ (Quanta Biosciences). The following primers were used: 5′-TAGTGTGTGCCCGTCTGTTG-3 (554-573) and 5′-CGCTTTCAAGTCCCTGTTCG-3′ (643-662) for the 5′ LTR; 5′-AAAGGGCTGTTGGAAATGTG-...
After incubation in darkness at room temperature for 20 minutes, samples were analyzed using flow cytometry (FACS verse; BD Biosciences, Franklin Lake, NJ, USA) and relevant FlowJo software. Biodistribution of PEG-DA-CuS NPs An aqueous solution (0.1 mL) of PEG-DA-CuS NPs (100 mg) was ...
Single cells were sorted by a BD FACS Aria II instrument (BD Biosciences). Meanwhile, mouse IgG1κ-FITC/PE isotype antibody (Southern Biotech, Birmingham, USA) was also used to establish the assay’s validity. The purity of the sorted cells was ≥ 98%, as analysed with FACSDiva ...
5). Therefore, either these tetraspanins were swept up into the budding virions by this specific location (Fig. 4) but have no function, or they are purposefully incorporated to perform a function for the virus (Fig. 7). In the light of our results, only CD81 appeared to be ...
These cells were then sorted by FACS with a BD FACSAria II system (BD Biosciences) to select GFP+ cells (indicating Addgene 48138 transfected). Sorted cells were plated into 96-well plates at a density of 1 cell per well and expanded. The genotypes of these isogenic cell clones were ...