Liver was homogenized in water and protein concentrations were determined by the BCA protein assay reagent (Pierce Protein Research Products; Thermo Fisher Scientific, Rockford, IL). Briefly, 20 μg of protein were incubated with 40 μl of 12.5 mM 4-methylumbelliferylsulfate substrate (4-...
For LCM analysis human as well as porcine RGC based MS raw files were combined and matched against the SwissProt human database using Proteome Discoverer version 1.1 (Thermo Scientific, Rockford, IL, USA) and MASCOT version 1.4.1.2 using a more relaxed strategy based on a 95% probability ...
22). The column system consisting of a 30 × 0.5 mm BioBasic C18 coupled to a 150 × 0.5 mm BioBasic C18 analytical column (Thermo Scientific, Rockford, IL, USA) and protected by an A316 online precolumn filter (Upchurch Scientific, ...