The objective of this study was to use a three-dimensional co-culture model of the blood vessel wall, comprised of ECs and MSCs, to determine how the presence of MSCs affects EC function.Hyeongseok KimMinsung HongHansu JoRandall F Ankeny...
1a)6, 7. While endothelial homeostasis is maintained in laminar shear stress (LSS) conditions, the prolonged exposure of the endothelium to oscillatory shear stress (OSS) caused by disturbed flow leads to the chronic inflammatory response, adversely increasing the risk of atherosclerosis and CVD ...
Response of the endothelial cell transcriptome to supraphysiological laminar shear stressResponse of the endothelial cell transcriptome to supraphysiological laminar shear stressWhiteS.J.HayesE.M.LehouxS.JeremyJ.ingentaconnectAtherosclerosis Amsterdam
The room has thermal homogeneity (laminar flow) with air flow maintained with independent heating and cooling systems capable of controlling room temperature over a range from 0° to 55°C. The application of two independent heating and cooling systems allows rapid changes in room temperature, up ...
Protein pellets were washed with 1 mL methanol (MeOH), centrifuged (21000 g/5 min/4 °C) and air-dried within a laminar flow hood for 10 min. Pellets, containing nucleic acids and proteins were solubilized in 1 mL TRIzol. Together with the lysing matrix B, samples were ho...
To determine mechanistic differences in the regulation of endothelial constitutive NO synthase (ecNOS), we measured NO production by bovine aortic endothelial cells exposed to shear stress in a laminar flow chamber or treated with Ca2+ ionophores in static culture. The kinetics of cumulative NO ...
The power-law fluid was injected, and its flow in the fracture is laminar or turbulent. The flow state of laminar and turbulent is described as follows: $$ \frac{\partial p}{{\partial s}} = - \alpha_{0} \frac{1}{{\overline{w}^{{2n^{\prime} + 1}} }}\frac{q}{{H_{fl}...
The current study evaluates the use of a custom designed fluid flow chamber's ability to study the role of cilia in regulating cell size in response to shear stress.A fluid flow chamber that continually maintains laminar flow at different flow rates and temperature was designed. Endothelial wild...
Upon continuous laminar shear stress application, ECs will change the alignment of actin filaments and microtubules to cause the alteration of cell shape and directional migration17,18. This process appears to be regulated by the Rac1-mediated signaling19, supported by the evidence that Rac1 was ...
In addition to nutriments deprivation, autophagy is also triggered by fluid-flow induced shear stress in epithelial cells, and this specific autophagic response depends on primary cilium (PC) signaling and leads to cell size regulation. Here we report that PI3KC2α, required for ciliogenesis and ...