The genome-wide transcription profile of Escherichia coli cells treated with hydrogen peroxide was examined with a DNA microarray composed of 4,169 E. coli open reading frames. By measuring gene expression in isogenic wild-type and oxyR deletion strains, we confirmed that the peroxide response ...
Hydrogen peroxide (H2O2) is produced predominantly in plant cells during photosynthesis and photorespiration, and to a lesser extent, in respiration processes. It is the most stable of the so-called reactive oxygen species (ROS), and therefore plays a crucial role as a signalling molecule in vari...
1886 spots were detected in the gels (Figure2). A set of 183 spots was analyzed further. 91 of them, representing 64 different proteins, were identified by MS (additional file1,MS_results.xls). The number of differently expressed proteins is similar to previous 2D-gel electrophoresis studies ...
(final concentration 1 M) or YPD supplemented with 2 mM hydrogen peroxide (final concentration 1 mM) and incubated for an additional 30 min. Cells were harvested by centrifugation and immediately flash-frozen in liquid nitrogen. Pellets were washed in protein extraction buffer (50 mM ...
Nox2, the most studied Nox, transfers an electron from NADPH to molecular oxygen to generate superoxide. Thus, this pathway is extremely sensitive to oxygenation status and cellular metabolism, which can rapidly change following infarct. Superoxide can then be rapidly converted to hydrogen peroxide ...
Cyanobacterial blooms are expected to increase, and the toxins they produce threaten human health and impair ecosystem services. The reduction of the nutri... Lürling,Miquel,Meng,... - 《Toxins》 被引量: 32发表: 2014年 EFFECT OF ELEVATED TEMPERATURE, DARKNESS, AND HYDROGEN PEROXIDE TREATMENT ...
transient production of high levels of ROS in response to external stimuli. Two major models depicting the origin of ROS in the oxidative burst are described, namely: the NADPH oxidase system and the pH-dependent generation of hydrogen peroxide by exocellular peroxidases. Additionally, the participat...
GO categories enriched for genes up-regulated in response to exogenous ABA or osmotic stress in roots and shoots include responses to light intensity, heat, wounding, and hydrogen peroxide (Additional File6). Additionally, genes enriched in GO categories such as response to stress, cold, and wate...
In addition, exposure to 200 μM hydrogen peroxide, which depletes HIF-1α accumulation in hypoxic cells and inhibits its ability to bind DNA,58 is associated with a 4-fold increase in TGF-β2 mRNA levels in hypoxic HUVECs and HepG2 (data not shown). Existing reports indicate a ...
The term ROS includes superoxide radical (O2−), hydrogen peroxide (H2O2), hydroxyl radical (OH), singlet oxygen, ozone, lipid peroxides, nitric oxide (NO) and peroxynitrite (ONOO−, also classified as a reactive nitrogen species (RNS) formed by the reaction of NO and O2−). Singlet...