The analysis by far-UV CD showed that the spectrum of recombinant F2-P27 protein was characterized by minima at 208–210 nm and at 218–220 nm consistent with a protein resembling a mix of alpha-helical and beta-sheet secondary structure. We also analyzed recombinant F2-P27 at different te...
LETM = longitudinally extensive transverse myelitis, relapsing ADEM* = multiphasic ADEM native extracellular P42S MOG (native-P42S1–117) was not altered by the point mutation, with the circular di- chroism (CD) spectrum characteristic of β-sheet fold- ing and similar to extracellular MOG (...
We then set out to determine experimental conditions that would provide a wide spectrum of different cytosolic siRNA release amounts, to use as the basis for subsequent dose-response analysis. The siRNA release amounts were similar for the two sequences as well as for an inactive control sequenc...
The Excel table below (attached to the calibration factsheet) reports the sensitivity values for the Q-MED in one-third octave bands. The dB value in Figure 1 is obtained from the sensitivity value in the one-third octave band and averaged RMS sensitivity as the dB reference (Figure 2)...
The file SLA_RI_Table_S9.xls is an excel file which contains two sheets. The "LPS" sheet contains the detailed information on differentially expressed genes after LPS stimulation, and the "PMA_ionomycin" sheet contains the detailed information on differentially expressed genes after PMA/ionomycin ...
SPF (WT, A; broad-spectrum antibiotics-treated, C) and GF (WT, B; 1 week after reconventionalization, D) TCRγδGFP reporter mice were anesthetized, and a small section of the ileum was prepared for in vivo imaging, leaving the rest of the bowel intact. Mice were injected with ...
In this study we have chosen to use five inbred mouse strains, with a spectrum of LPS susceptibility as genetic tools to determine the nature and diversity of innate immune responses to a single pathogenic stimulus. The strains chosen (C57Bl/6J, DBA2, BALB/c, C3H/ARClps nand C3H/HeJlps...
activated genes during heat shock in somatic cells, we observed only moderate HSF1 binding to the promoters in hepatocytes (and no binding in spermatocytes). In further in-depth ChIP on chip studies our goal was not study of the kinetics of HSF1 binding but finding the broadest spectrum of ...
and mutations in this gene cause autism spectrum disorder and schizophrenia.61,62 It is noteworthy that the nearby 22q11.2 deletion syndrome [22qDS/velocardiofacial syndrome (VCFS)/DiGeorgio syndrome/CATCH22] is one of the most common multiple anomaly syndromes in humans and it also includes am...
developmental delay, intellectual disability, delayed speech and seizures, and involves theSHANK3gene.60TheSHANK3gene encodes a protein that has a role in synapse formation and dendritic spine maturation, and mutations in this gene cause autism spectrum disorder and schizophrenia.61,62It is noteworthy...