(Chroma Corporation) for CFP, YFP, and FRET (CFP excitation and YFP emission). Images were collected on a 14-bit Cascade HQII digital camera and were processed as described in Hoppe et al. to determine the FRETstoichiometryof the interaction between PLD2 and Rac2 (Hoppe, Christensen, & ...
We recently developed a series of FRET-based technologies to determine protein interaction dissociation constant and for use in high-throughput screening assays of SUMOylation. SUMO (small ubiquitin-like modifier) is conjugated to substrates through an enzymatic cascade. This important posttranslational ...
Mallard CLoeliger MCopolov DRees S Reduced number of neurons in the hippocampus and the cerebellum in the postnatal guinea-pig following intrauterine growth-restriction. Neuroscience 2000;100327- 333PubMedGoogle ScholarCrossref 62. O'Brien JT The “glucocorticoid cascade” hypothesis in man: prolonge...
Although the causative origin of these changes is known for individuals with ADAD, the mechanisms that trigger this cascade of events in sporadic AD are less clear and clearly multifactorial. Nevertheless, we propose that, in concert with the above-mentioned strengths of studying ADAD cohorts, ...
Hyperbranched polymers named dendrimers (also known as cascade polymers54) are synthetic polymers in the form of a globular structure with numerous arms radiating from a central core.131,132 Biomedical applications require the dendrimer backbones to be water soluble and biocompatible for use in drug ...
Capturing a rhodopsin receptor signalling cascade across a native membrane Article Open access 06 April 2022 Label-free, real-time monitoring of membrane binding events at zeptomolar concentrations using frequency-locked optical microresonators Article Open access 28 August 2024 Introduction G-protein...
As WMA develop late in the ischemic cascade, myocardial strain analysis allows earlier detection of ischemia-induced myocardial dysfunction: a recent FT study [58] showed that, despite no differences at rest, at high-dose dobutamine, circumferential strain was significantly impaired in territories ...
(i.e., blast versus focal versus diffuse) may be an important factor to consider as this could affect the consequent pathophysiological cascade. Unfortunately, the absence of a specific phospho-tau antibody suitable for RPPM at the time of the study prevented us from determining the effect of ...
These electrons are then attracted to the second dynode, generating more electrons, and so on, in a cascade through the device. Negative ions may be detected by using a conversion dynode held at a high positive potential. Channeltron detectors operate according to similar principles as discrete ...
were collected to assemble the normal control cohort without CVDs, allowing us to develop and validate the noninvasive screening model. Table1and Extended Data Table1contain the summary statistics and the demographics of the datasets. The inclusion–exclusion cascade is summarized inMethodsand Extended...