first person to define the term numerical aperture. In 1873, Abbe published his theory and formula which explained the diffraction limits of the microscope[2]. Abbe recognized that specimen images are composed of a multitude of overlapping, multi-intensity, diffraction-limited points (or Air...
emission electron microscope (EEM)magnetic fieldresolutionThe known Br眉che-Recknagel formula for determining the resolving power of an emission electron microscope (EEM) was derived assuming idealized conditions: the object surface was planar, and the accelerating electric field was homogeneous. However,...
simulations showed that the resolution of the reconstruction would be limited to a lower resolution if the images were from a microscope with lower acceleration voltage. Based on these results, a modification from a previous empirical formula21: ...
After performing the second filtration with a cell strainer, the single-cell suspension was stained with fluorescein diacetate (FDA) at 14 mM final concentration for 3 min on ice and the number and activity of cells was calculated with microscope (ZEISS Axioscope A1). The protoplasts with a...
Owing to its unique penetrating power and high-resolution capability, X-ray imaging has been an irreplaceable tool since its discovery. Despite the significance, the resolution of X-ray imaging has largely been limited by the technical difficulties on X-
The resolution of optical microscope and electron microscope is discussed briefly first. 简述了光学显微镜与电子显微镜的分辨本领 。 更多例句>> 3) resolution power 分辨本领 1. The effect of the nonlinear terms on the resolution power and measuring accuracy of the circular mass spectrometer is analy...
Microscope Parameters Objective:4x / 0.51 NA Total Magnification:4.00 x Lateral Resolution:608.80 nm Axial Resolution:3913.88 nm Sampling Rate (Nyquist Sampling):0.41rlat/ pix Depth of Field:872.52 nm Depth of Focus:13.96 µm Brightness:42.28...
‘zebrafish single-cell atlas of perturbed embryos’: single-cell transcriptomic data from 1,812 individually resolved developing zebrafish embryos, encompassing 19 timepoints, 23 genetic perturbations and a total of 3.2 million cells. The high degree of replication in our study (eight or more ...
The development of high-resolution microscopes has made it possible to investigate cellular processes in 3D and over time. However, observing fast cellular dynamics remains challenging because of photobleaching and phototoxicity. Here we report the imple
A sharp point source will appear smeared out and have the shape of the point spread function (PSF). Interpreting an observed digital scene as a very fine grid of point sources, a digital microscope image will be a discrete convolution of these sources with the PSF, which is often ...