MSSR is tailored around the assumption that fluorescence images are formed by discrete signals collected (photons) from point sources (fluorophores) convolved with the PSF of the microscope (Supplementary Notes1,2and3). Processing a single image with MSSR starts with the calculation of the MS, w...
Ernst Abbe theoretically derived the fundamental limit of an optical microscope, stating that the resolution of a microscope is limited by the numerical aperture of the objective and
This definition applies to biological super-resolution microscopy as well. However, what qualifies as “super-resolution” for microscopy images is strictly defined by the diffraction limit of ~200 nm. In contrast, what qualifies as “super-resolution” for natural images is more loosely defined ...
Quantitative Imaging in Cell Biology Jennifer C.Waters,TorstenWittmann, inMethods in Cell Biology, 2014 1.5.12D sampling As discussed in the preceding text, resolution of the optical image generated by the microscope is limited by thePSF. Resolution in the digital image (on which we make our ...
While the x-ray microscope will not likely allow full satisfaction of this dream, it does offer new and exciting possibilities in this direction. In what follows we will consider the basis for this view both in general and by means of a specific example, the important and ubiquitous cellular...
Candidate spot positions were identified as local maxima of 3D frames filtered by a 3D Gaussian filter matched to the point-spread-function (PSF) of the microscope. To distinguish between local maxima originating from signal and noise, respectively, we used unimodal thresholding of the estimated ...
Definition: the optical component of a microscope which is next to the observed object More general term: objectives Categories: vision, displays and imaging, optical metrology DOI: 10.61835/0vk Cite the article: BibTex plain textHTML Link to this page LinkedIn The microscope objective is a key...
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Single-molecule dynamic structural biology with vertically arranged DNA on a fluorescence microscope Graphene energy transfer with vertical nucleic acids (GETvNA) is a general approach enabling dynamical observations of DNA structural changes and DNA–protein interactions with spatial resolution down to the...
RESI approaches structural biology resolution with an all-optical approach in intact cells using off-the-shelf labelling reagents and a simple inverted fluorescence microscope operated under ambient conditions. We were able to experimentally demonstrate Ångström spatial resolution below the physical size...