Cells were fixed with 1% formaldehyde for 10 min at room temperature before termination with 0.125 M glycine. Cells were then lysed in ChIP buffer (0.3% SDS, 300 mM NaCl, 1 mM EDTA, and 50 mM Tris-HCl pH 8.0), and cross-linked chromatin was sonicated to obtain DNA fragments around 250...
Once the system converged, a two-step equilibration (each 100 ps) was applied to ensure the correct temperature and pressure of the solvated system was attained. The temperature was set at 300 K (NVT – constant number of particles, volume and temperature) using the Berendsen thermostat. ...
Biodiversity often helps communities resist invasion. However, it is unclear whether this diversity–invasion relationship holds true under environmental changes. Here, we conduct a meta-analysis of 1010 observations from 25 grassland studies in which pl
Temporal changes in water temperature and salinity drive the formation of a reversible plastic-specific microbial community. FEMS Microbiol Ecol. 2020;96:1–10. https://doi.org/10.1093/femsec/fiaa230. Article CAS Google Scholar Pinnell LJ, Turner JW. Shotgun metagenomics reveals the benthic ...
MTL-1 is known to be upregulated by elevated temperature46, although its role in the heat shock response remains unclear. We found that the biofilm-mediated increase in thermotolerance was abrogated inmtl-1knockout worms (Fig.4b), thus implicating MTL-1 in biofilm-mediated heat resistance. Cur...
[32]. Genomic DNA was PCR-amplified using 200 μM of each dNTP, 1 U ofTaqpolymerase, and 2.5 pmol of each primer at an annealing temperature of 55°C with 30 PCR cycles. PCR products were run on a Beckman Coulter CEQ 8000 capillary sequencer and alleles were sized with the CEQ 8000...
were plotted as bar plots and line graph (Additional file3: Fig. S2). DHs across environments showed a gradual increase in PHS resistance with increasing number of QTLs (Additional file3: Fig. S2). Notably, a highly resistant phenotype was observed only when at least five major QTLs were ...
A longitudinal analysis of methicillin-resistant and sensitive Staphylococcus aureus incidence in respect to specimen source, patient location, and temperature variation Int J Infect Dis, 54 (2017), pp. 50-57 View PDFView articleView in ScopusGoogle Scholar 65 R Oldenkamp, C Schultsz, E Mancini,...
(c). Furthermore, similar to LSR, GNDs were accumulated towards the substructure networks. Thus, columnar and cellular microstructure improved the creep resistance at high temperature by suppressing the dislocation movement; (e) graph between creep strainvscreep time depicting different stress levels ...
FIG. 4 is a graph of effective thermal capacitance of the weld volume vs. temperature, FIGS. 5a and 5b are idealized curves for weld resistance and rate of resistance change, FIGS. 6a and 6b are representative curves for weld resistance and rate of resistance change, ...