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While T-cell responses to cancer immunotherapy have been avidly studied, long-lived memory has been poorly characterized. In a cohort of metastatic melanoma survivors with exceptional responses to immunotherapy, we probed memory CD8+ T-cell responses acr
Cutting edge: gut microenvironment promotes differentiation of a unique memory CD8 T cell population. J. Immunol. 176, 2079–2083 (2006). Article CAS PubMed Google Scholar Hoekstra, M. E., Vijver, S. V. & Schumacher, T. N. Modulation of the tumor micro-environment by CD8+ T cell-...
Department of Molecular and Cellular Hemostasis, Sanquin Research and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands Teunis J. P. van Dam Department of Microbiology and Immunology, Renaissance School of Medicine, Stony Brook University, Stony Brook, NY, USA ...
The authors thank patients with coeliac disease and their family members, as well as the University of Chicago Coeliac Disease Center, for supporting their research. The authors also thank L.B. Barreiro and B. Sally for critical reading of the manuscript. The work was supported by grants from...
Jacobs University Bremen GmbH, Bremen, Germany Angélique Gobet Josephine Bay Paul Center, Marine Biological Laboratory, Woods Hole, MA, USA Susan M Huse & Mitchell L Sogin Alfred Wegener Institute for Polar and Marine Research, List/Sylt, Germany Justus E E van Beusekom School of Engineering, ...
Skeletal muscle cells were isolated by a standard method.48 Briefly, skeletal muscle was minced into 1 mm3 fragments and digested at 37 °C, first using Liberase (Roche Diagnostics, Meylan, France, http://roche.fr/portal/eipf/france/rochefr/ recherche/home) for 1 h and then using 0.25% ...
GBIF data portal (2011). Royal British Columbia Museum; Ontario Breeding Bird Atlas 2001–2005; Macaulay Library—Audio Data, Bird Collection; Peabody Ornithology DiGIR Service, Bird Tissue Collection; University of Alberta Museums, Ornithology Collection; Project FeederWatch; eBird Bird Observation ...
Briefly, mice were anaesthetized and the portal vein was cannulated. The liver was first digested with 0.5 mM EGTA and then digested with 0.05% collagenase IV, the digested liver resuspension was passed a 200-gauge steel mesh and the hepatocytes in the flow through were isolated by 40% ...
K. et al. Cutting edge: CD69 interference with sphingosine-1-phosphate receptor function regulates peripheral T cell retention. J. Immunol. 194, 2059–2063 (2015). Article CAS PubMed Google Scholar Wu, T. et al. Lung-resident memory CD8 T cells (TRM) are indispensable for optimal cross...