Enhancers, as a class of non-coding DNA regulatory elements, are composed of clusters of transcription factor (TF) binding sites and specifically regulate the expression of their target genes through enhancer-promoter interactions. Whole genome sequencing methods have shown that more than two million ...
The dried peptides were cleaved of the biotin portion of the ICAT reagent with the cleaving reagents at 37 °C for 2 h. Then the ICAT-labeled peptides were dried by vacuum centrifuge and resolubilized in 0.1% formic acid for 2D-LC-MS/MS analysis. 2D-LC-MS/MS— Orthogonal 2D-LC-MS/...
Classically, enhancers and promoters have been defined as regulatory elements with different functions that can be clearly distinguished based on their position and length. The continuous development of high-throughput sequencing technology has enabled large-scale and in-depth studies of enhancers and prom...
6 C). In agreement with this, higher enrichments of miR-145-5p and Ago2 were observed in the biotin-labeled LNCAROD group than the control (Fig. 6D). These data suggest that LNCAROD acts as a molecular sponge for miR-145-5p in HCC via the ceRNA mechanism. Fig. 6 miR-145-5p ...
In the study of tumor pathogenesis, scRNA-seq technology further verified the molecular mechanisms of tumor genesis by analyzing transcriptomics of each cell subgroup, as well as for rare cell clones. scRNA-seq shows that one or more important gene mutations occur in a few somatic cells, which ...
Also, MSC spheroid formation generated by the Biotin-DFYIGSR hydrogel can eliminate and scavenge ROS in HUVECs and RAW264.7 cells, as well as in diabetic wounds [66]. Jiao et al. designed a combination therapy for diabetic wound healing using Wharton’s jelly MSCs embedded in PF-127 ...
Also, MSC spheroid formation generated by the Biotin-DFYIGSR hydrogel can eliminate and scavenge ROS in HUVECs and RAW264.7 cells, as well as in diabetic wounds [66]. Jiao et al. designed a combination therapy for diabetic wound healing using Wharton’s jelly MSCs embedded in PF-127 ...
Severe COVID-19 disease caused by SARS-CoV-2 is frequently accompanied by dysfunction of the lungs and extrapulmonary organs. However, the organotropism of SARS-CoV-2 and the port of virus entry for systemic dissemination remain largely unknown. We profiled 26 COVID-19 autopsy cases from four...
Brain metastasis (BM) is common among cases of advanced non-small cell lung cancer (NSCLC) and is the leading cause of death for these patients. Mesothelin (MSLN), a tumor-associated antigen expressed in many solid tumors, has been reported to be involve
After washing, 2 mL of Biotin-Conjugated Anti-Cytokines was incubated with membranes overnight at 4 °C. Then, 2 mL of HRP-conjugated streptavidin was added to the washed membranes and incubated overnight at 4 °C. Finally, 500 μL of detection buffer was added to the membranes for 2 ...