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Single-cell RNA-seq is an unbiased approach that has extended our understanding of heterogeneous tissues, including mouse and human embryonic gonads.18,19,20We reasoned that analysis of stage-specific gene expression profiles of individual spermatogenic cells could provide unbiased and novel insights int...
The consumption of junk foods, fast foods, sugar-sweetened beverages, and carbonated drinks and beverages is associated with higher body mass index in children and adolescents due to their high content of free sugar and energy (Gupta et al.2019). In order to promote public health sensitizing th...
Next day, the sections were washed 3 times in PBS, and incubated with the appropriate secondary fluorescent antibodies for 1 h at 25 °C (AlexaFluor goat anti-mouse IgG (1:500, Invitrogen) or Alexa Fluor goat anti-rabbit IgG (1:500, Invitrogen). Cell nuclei were visualized with ...
Rabbit IgG was used as the negative control. The primer sequences are shown in the Methods section. The enriched DNA fragments were subjected to PCR and DNA gel electrophoresis. K, L YAP silencing decreased binding to the promoter region of the CXCR7 gene. YAP was depleted in MGC803 and ...
Next, TMAs were incubated with biotin-conjugated secondary antibody for 30 min (Goat-anti-Rabbit, RRID: AB_3073814, Vectastain, Vectorlabs, Burlingame, CA), followed by incubation with avidin-streptavidin complex (Vectastain, Vectorlabs, Burlingame, CA) for 30 min. Sections were developed with 3...
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Re-activating quiescent adult epicardium represents a potential therapeutic approach for human cardiac regeneration. However, the exact molecular differences between inactive adult and active fetal epicardium are not known. In this study, we combined fet
After, the blots were incubated with goat anti-rabbit 800 antibodies (1:5000) for 2 h at room temperature. The immunoreactive bands were visualized by a two-color infrared imaging system (Odyssey; LI-COR, Lincoln, NE, USA). Statistical analysis GraphPad prism V5.0 software (Graphpad prism...