@文心快码BaiduComate"docker run" requires at least 1 argument(s). see 'docker run --help'. usage 文心快码BaiduComate 解释"docker run"命令的基本用法 docker run命令用于从指定的镜像创建一个新的容器,并在容器内执行命令。它是Docker中最常用的命令之一,用于启动和管理容器。 说明"docker run"命令至少...
Measurements were only validated for a given spindle if at least five different values could be obtained. The lagging chromosome frequency was assessed in fixed HeLa cells stained with DAPI (1 μg/ml; Sigma-Aldrich) diluted 1:1000 to detect DNA and centromeres with an anti-CREST antibody ...
2 and Supplementary Table 2 and are the mean±s.d. from at least three independent replicates. Statistical analyses and P-values for the kcatapp and Kmapp values from a and b are listed in Supplementary Table 11. Reciprocal Dalziel coefficients for d were obtained from Supplementary Fig. 3 ...
The Pro residues of HIV-1NL4-3 Vpr are located in the N-terminal domain of the protein, namely at positions 5, 10, 14 and 35. All previously determined binding domains of various proteins interacting with CypA have hitherto required at least one Pro residue as part of the binding region....
Hits were considered positive when at least two peptides with more than six residues and 90% confidence were detected. Results In silico analysis The genome-scale model, iJO1366, predicts that PA can be natively produced The E. coli GEM, iJO1366 [26] was used to test the native metabolism...
Well now at least scripts with chinese text string work. But if there is somewhere some 'RawBinaryString' like $RawData = "??#$?%H" this string data will get corrupted. Workaround: To fix that open the file in SCiTE and choose File/Encoding/UTF8 and save it(may change sth to be ...
Pre-polymer solutions were mixed thoroughly for at least 5 minutes, degassed, and spin-coated at 5000 rpm on 25 mm glass coverslips. PDMS was then cured for 2–3 hours at 60 °C. Samples were stored at room temperature in a vacuum desiccator. Measurement of polyacrylamide and ...
1a) and found that, although 62.3% TOP1cc proved to locate with Top1-enriched regions (Extended Data Fig. 1b–d), TOP1cc could be detected at only around 14.1% of the Top1-enriched regions detected with ChIP–seq29, which might reflect assay noise due to the formaldehyde fixation17. ...
Briefly, His-CbpA served as bait and was incubated with HisPur Cobalt Resin at 4 °C for at least 30 min, the resin was washed with wash solution, and incubated with HT-29 cell lysates overnight at 4 °C. HT-29 cell lysates were prepared according to the user guide of the ...
At least 20 cells per condition were imaged using the same exposure settings, to allow quantitative analysis of GFP intensity in the host cell (signal outside the parasite and PV) between chimeras. All statistical analyses were performed using Prism 6 and mean differences were compared using the...