The invention of the microscope led to the explosive development of biological research, including brain research. After Ernst Abbe’s diffraction limit (λ/2, where λ is the wavelength of light) was published in 1873, Stefan W. Hell developed stimulated emission depletion microscopy, which overca...
Live-cell confocal image acquisition was performed using a Leica SP8 confocal microscope system with a 40X, 1.30NA oil-immersion objective, equipped with a heated, humidified stage top incubator with 5% CO2 and 37° C (Tokai Hit, STX stage top incubator). To observe progression through cytok...
Intravital imaging was performed on a two-photon microscope (Sutter MOM) with a 920 nm excitation light (Mai Tai eHP Deep See, Spectra-Physics). Green fluorescence emission (Chroma 565 dcxr) was gathered using a 40× infrared objective (Olympus, 0.8 NA). ScanImage software (Pologruto et ...
In this New Moon chart, Saturn the planet most invested in practical application is positioned at the mid-point of the Mercury/Neptune sextile. It intercepts the dream and urges one to focus on results. Mercury in Capricorn is also highly result oriented. Neptune in Pisces says, ‘there are...
Cells expressing an mChe-fused ICK construct were serum-starved for 24 h on a glass-bottomed culture dish, placed on a microscope stage prewarmed to 37 °C, and observed using a TIRFM ECLIPSE Ti microscope (Nikon) at a video rate using the NIS-Elements imaging software (Nikon). Data ...
The analysis of the MEs of wild-type and mutant cells in the electron microscope revealed that the ESCRT-containing electron-dense microdomains of ILV formation at the limiting membrane are elongated, indicating a change in ESCRT activity. Since lgd mutants can be rescued to normal adult flies ...
Samples were then stained with 2% uranyl acetate and were observed using a Tecnai T12 electron microscope at an accelerating voltage of 100 kV. Images were taken digitally with a Teitz TCL camera system. Pili conductivity. As previously described (11), for conductivity mea- surements, pili ...
Knock-in (KI) mouse models of Alzheimer’s disease (AD) that endogenously overproduce Aβ without non-physiological overexpression of amyloid precursor protein (APP) provide important insights into the pathogenic mechanisms of AD. Previously, we reported
These results highlight a genetic and molecular link between the effect of the snoRNA on ribosomal RNA, some downstream deregulated genes and lifespan. Jouvence modulates the resistance to various stresses Several stress-response genes have been reported to influence lifespan5,14. Moreover, several ...
Aniline blue staining was performed as described previously17, and the samples were analysed with a Leica DM6000B microscope (Leica Microsystems). For GWAS phenotyping, 9–20 siliques of a minimum of three individuals were analysed for each accession (Exceptions: Zdr-1: seven siliques, Got-7:...