Center for Vaccines and Immunity to Microbial Pathogens, Washington University School of Medicine, St. Louis, MO, USA Michael S. Diamond Contributions S.R.M. performed binding and neutralization assays, immunization, passive transfer, depletion studies, challenge experiments and flow cytometry. P.D....
Blood was collected from marginal ear vein on 35th day for immunob- lotting and afterwards 3rd booster and further immunizations were performed after periodic intervals. The exper- iment was terminated after 60 days and blood was collected. Antibodies were purified using antibody purification kit ...
Governor Roy Cooper proclaimed August as Immunization Awareness Month in North Carolina. Alongside the proclamation, NCDHHS is partnering with health care providers and stakeholders in a statewide awareness campaign to help e...
Anti-KSHV Rta (replication and transcription activator) antibody was generated by immunization of rabbits with ORF50 peptide (amino acids 667-691) [8]. 2.3. Western blot analysis After infection, cells were harvested and lysed in RIPA buffer containing protease and phosphatase inhibitors. 60-80 ...
Anti-KSHV Rta (replication and transcription activator) antibody was generated by immunization of rabbits with ORF50 peptide (amino acids 667-691) [8]. 2.3. Western blot analysis After infection, cells were harvested and lysed in RIPA buffer containing protease and phosphatase inhibitors. 60-80 ...
(e) Graphical representation of the frequencies of antigen-specific IgG1 cells in immunized spleens. (f) Graphical representation of the frequency of antigen-specific IgG1 cells at day 10, 14 and 28 post immunizations. Cells were gated as in (d). Group of five mice were used, and data ...