Variants at 90,409 loci were observed to be present among at least 1% of alleles. As expected, polymorphisms in essential genes are slightly less common than those in non-essential genes (median minor allele frequency: essential = 0.141, non-essential = 0.146; p = 0.005, one-...
Here, we show that macropinocytosis is universally induced by hypoxia in all HCC cell lines tested. Hypoxia is frequently observed in solid cancers, including HCC. As hypoxic regions are poorly perfused and have limited nutrients, we posit that hypoxic cancer cells should have a greater reliance o...
(Basel, Switzerland). MiSeq analysis was performed to genotype the loci that were targeted by the CRISPR RNP complex and showed 94% allele mutation frequency. Loss of Rictor and Raptor protein expression was confirmed using Western blotting. The gRNA sequences and primers used are given in ...
In accordance with these observations, we consistently detected decreased levels of cyclin D1 in ML364-treated cells and in an AU565 stable cell line in which we successfully knocked out one USP2 allele using CRISPR/Cas9 technology and observed enhanced ErbB2 downregulation following 17-AAG ...
The data so far suggested that HER2(776–788) is presented in the context of HLA-DR and in particular of the HLA-DRB5*0101 allele which was shared between the FM3 melanoma cell line and the donor of the T cell line (donor 1; Table 1). However, this particular DR-allele was not ...
Minnucci, G.; Amicarelli, G.; Salmoiraghi, S.; Spinelli, O.; Guinea Montalvo, M.L.; Giussani, U.; Adlerstein, D.; Rambaldi, A. A novel, highly sensitive and rapid allele-specific loop-mediated amplification assay for the detection of the JAK2V617F mutation in chronic myeloproliferative...
In the healthy organoids, the TROP2 expression levels were quite similar and increased with the stimulation of the Wnt pathway. The same trend was observed for the Wnt target genes and vimentin. In contrast, the increasing stimulation of the Wnt pathway resulted in the decreased expression of ...