Reporter genes can also serve as controls for transfection. For example, transfection efficiencies between different experiments can be normalized by comparing the expression levels of a reporter gene used in all of the experiments. Gene regulation assays ...
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Example Sentences Examples have not been reviewed. Historically, when researchers needed to determine where to target their edits, they would typically manually screen for potential integration sites, then test the site by integrating a reporter gene to assess its cellular fitness and gene expression l...
Optimize your reporter gene assay experiments to get the best results. We’ve compiled a detailed knowledge base of the top tips and tricks to meet your research needs. View the relevant questions below: Having proble...
A good example for this is the original description of the chloramphenicol acetyltransferase (CAT) assay in yeast. The authors of this study demonstrated the validity of CAT as a yeast reporter gene by constructing vectors that contained the coding region of the bacterial cat gene, but no ...
Reporter gene assays have been carried out in a variety of cell types containingER, either endogenously or with prior transfection of ERα/ERβ into the cells. For specific human health considerations, it is most relevant to use human cells from the target organ of interest. For example, effe...
Bioluminescent reporter gene assays have a distinct advantage over fluorescent assays, such as green fluorescent protein (GFP), in that they can deliver 10- to 1,000-fold higher assay sensitivity. This greatly increased sensitivity can substantially improve assay performance when applied to complex bio...
Phophatases hydrolyze a variety of organic phosphate monoesters to alcohols and inorganic phosphate. This important class of enzymes is involved incellular signal transduction,cell metabolismand other biological activities. Some phosphatases serve as enzyme markers. For example,PALP-1, the gene for huma...
The reporter mouse model is useful in monitoring the in vivo transcriptional regulation of the interested gene, they have a wide range of application prospects in cancer therapy, drug discovery, etc. For example, Cytochrome P450 3A4 (CYP3A4) plays an important role in drug metabolism, and the...
Reporter gene assays are widely used to study cellular signaling and transcriptional activity. Few studies describe the use of reporter genes for studying cellular responses on complex body fluids, such as urine and blood. Selection of the optimal report