Parcelization of geographic data for storage and use in a navigation application A system and method for arranging and storing a plurality of records of geographic data, wherein each record corresponds to a physical feature having a physical location in a geographic region. The method and system ...
The coordination of transcription and timing of DNA replication during the S phase of the cell cycle has recently been studied chromosome-wide in high reso... M Schwaiger,D Schubeler - 《Current Opinion in Genetics & Devolopment》 被引量: 151发表: 2006年 Location, location, location: it's...
Recently, replication timing has been analyzed by single-cell sequencing of several hundred mouse or human cells15,16,17. These studies focused on cells in the middle of S phase and analyzed replication at the level of domains, concluding that stochastic variation exists in replication timing and...
also observed in individual cell lines, the latter when extending the overlapping regions by 1 kb on each side of the modification peaks (Figs.S6andS7). Thus, these modifications occurring in close proximity to each other may mark the locations of replication initiation sites. We note, however,...
In the novel replication mechanism of closed circular mouse L-cell mitochondrial DNA synthesis one strand of the duplex (the heavy-strand) is initiated at a defined origin and proceeds unidirectionally. Synthesis of the complementary light-strand is initiated at a different origin, located approximatel...
Cell proliferation in the imaginal wing disc of Drosophila has been analyzed by both pulse and chronic labeling with [ 3H]thymidine. We find neither spatial nor temporal variation in the fraction of S phase cells during the third instar. At or near the time of white prepupae formation the fr...
us to determine RFD genome-wide in asynchronous cultures of two human cell lines, without any genetic modification. We infer the location of replication initiation and termination events at high resolution and paint a novel, comprehensive landscape of human genome replication in these two cell types...
DNA replicates once per cell cycle. Interfering with the regulation of DNA replication initiation generates genome instability through over-replication and has been linked to early stages of cancer development. Here, we engineer genetic systems in buddin
In eukaryotic cells, segments of chromosomes replicate via the synchronous firing of clusters of replication origins [1]. These segments or "replication domains" replicate in a defined temporal order during S-phase. This replication-timing program is cell type specific [2], and developmentally regula...
Pu–seq provides direct replication origin location and efficiency data and indirect estimates of replication timing. ... Y Daigaku,A Keszthelyi,CA Müller,... - 《Nature Structural & Molecular Biology》 被引量: 99发表: 0年 De?novo identification of replication-timing domains in the human ...