Detailed analysis of the radioactivity profiles obtained on sedimentation of the resulting labelled DNA fragments in alkaline sucrose gradients has provided information about the size of the growing DNA strands. The results suggest that most of the replicating units have a single-strand molecular weight...
DNA double-strand breaks measured in individual cells subjected to gel electrophoresis. However, for the very radiosensitive L5178Y-S cells, the migration of replicating DNA was reduced only slightly. For Chinese hamster V79 and Chinese hamster ovary cells, damage was repaired at a similar rate in...
SSB proteins (single strand binding) keep DNA from folding in on itself & keeps any secondary structures form forming -->snap & replication would NOT continue DNA gyrase Enzyme that temporarily breaks the strands of DNA, relieving the tension caused by unwinding the two strands of the DNA helix...
Two DNA repair pathways are known to mediate DNA double-strand-break (DSB) repair: homologous recombination (HR) and nonhomologous end joining (NHEJ). In a... Decottignies,A. - 《Genetics》 被引量: 193发表: 2007年 Pharmacological characterization of glutamatergic agonists and antagonists at rec...
Careful mapping of initiation sites revealed a precise transition from leading to lagging strand synthesis at ARS1 between the B1 and B2 elements [24]. B2 was originally proposed to be a DNA unwinding element based on studies that showed functional substitution of the element with other eas...
J. Stability of the human polymerase delta holoenzyme and its implications in lagging strand DNA synthesis. Proc. Natl Acad. Sci. USA 113, E1777–1786 (2016). Article ADS CAS Google Scholar Sakurai, S. et al. Structural basis for recruitment of human flap endonuclease 1 to PCNA. EMBO J...
The dut mutants of Escherichia coli fail to hydrolyze dUTP and thus incorporate uracil into their DNA, suffering from chromosomal fragmentation. The postulated mechanism for the double-strand DNA breaks is clustered uracil excision, which requires high density of DNA-uracils. However, we did not fi...
Induction and repair of double-strand breaks in the replicating DNA of HeLa cells. The induction and closure of double-strand breaks produced by X rays were measured in the replicating DNA of HeLa S3 cells using the techniques of neutral ... RL Warters,BW Lyons - 《Radiation Research》 被引...
8.Sequencing-by-synthesis methods simulate parts of this process on a single DNA strand of interest.合成定序法模拟细胞的复制步骤,分析单股DNA的序列。 9.Duplicate column name resolution could not be done because the ordinal specified a column of a different name.因为序号指定了具有不同名称的列,所...
The buoyant density in caesium chloride of the replicating DNA was found to be 5 to 10 mg/cm 2 greater than that of mature adenovirus type 2 DNA. The single-strand specific nuclease from Neurospora crassa released 25 to 30% of the radioactivity from replicating DNA and the density difference...