Endpoint ELISA titers normalized using these curves showed a similar range of anti-TNP reactivity between FLAG+ and Strep+ fractions (Extended Data Fig. 2e), indicative of equal competitiveness of the differently tagged B cells. Following the serum antibody produced by B cells engaged at different...
10 µl/ml Pen/Strep (Invitrogen), and 10 µl/ml Glutamax (Invitrogen). The cells were seeded on 6-well plates (Falcon/Olympus) at 1,000,000 cells/well in a volume of 2 ml per well. Cells were also seeded on 4-chamber CC2 glass slides...