COMPOSITE platesARTIFICIAL neural networksPOISSON'S ratioSTRAINS & stresses (Mechanics)This article explores the use of analytical data and machine learning algorithms to improve the performance of damage repair in thin-walled structures, specifically focusing on composite materials used in...
Initially, BMSCs were grown on the surfaces of the hydrogels in 24-well plates at a density of 5 × 104 cells per well. After 7 days of culturing, the samples were stained using an ALP staining kit (Yeasen) and observed. Simultaneously, cell protein was extracted with a total cell...
All cells were cultured with 5% CO2 in a 37 °C incubator. Cell viability assay Cell viability was assessed by crystal violet assay. Cells were plated in 24-well plates with a cell density of 4 × 104 cells per well, and incubated at 37°Cfor 24 h. Then the cells were treated ...
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To assess cell viability, cells were plated at a density of 3000 cells/well in 96-well plates. After 24 h, the cells were treated with different concentrations of drugs for 48 h. Before harvest, we replaced the medium with fresh medium containing MTT (0.5 mg/ml), and then the...
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This cell suspension was further diluted to OD600 = 0.1 with YPD (for −MMS control cultures) or 0.6% MMS (v/v) in YPD (for +0.3% MMS cultures). Cell suspensions were incubated at room temperature with rotation for 1 h before diluted aliquots were spread on YPD plates. Colonies were...
Plates were considered valid when at least 2/3 of the quality controls were within 30% of the expected values. Western blots Mouse cortical neurons were lysed in RIPA buffer containing a protease inhibitor cocktail. Samples were denatured at 95 °C for 5 min in a 6X Lamelli buffer (...
Cells from passages 3 to 5 were used for subsequent in vitro experiments. Analysis of colony-forming unit fibroblast (CFU-F) CBSCs were seeded into 6-well plates at a density of 200 cells/well and cultured for 12 days. When visible colonies were formed, the colonies were fixed with 20%...
Four different concentrations of hUCMSC-derived exosomes (0, 10, 50, and 100 μg/mL) were applied to evaluate the impact on HUVEC proliferation. In brief, HUVECs (2000 cells/well) were seeded into 96-well plates in EGM-2 (Lonza) and cocultured with different concentrations of exosomes (...