COMPOSITE platesARTIFICIAL neural networksPOISSON'S ratioSTRAINS & stresses (Mechanics)This article explores the use of analytical data and machine learning algorithms to improve the performance of damage repair in thin-walled structures, specifically focusing on composite materials used in ...
Initially, BMSCs were grown on the surfaces of the hydrogels in 24-well plates at a density of 5 × 104 cells per well. After 7 days of culturing, the samples were stained using an ALP staining kit (Yeasen) and observed. Simultaneously, cell protein was extracted with a total cell...
Mineral trioxide aggregate (MTA) is considered a bioactive endodontic material, which promotes natural mineralization at the material-tooth tissue interface. MTA Repair HP stands out because of the short setting time and the quick and effective bioactive response in vitro. The bioactivity, depens on...
This cell suspension was further diluted to OD600 = 0.1 with YPD (for −MMS control cultures) or 0.6% MMS (v/v) in YPD (for +0.3% MMS cultures). Cell suspensions were incubated at room temperature with rotation for 1 h before diluted aliquots were spread on YPD plates. Colonies were...
To stably integrate the BFP sequence, 6 × 105 cells were infected with lentivirus at MOI of 0.3 in 6-well plates (Corning, NY, USA) containing DMEM supplemented with 10% FBS and 8 μg ml−1 polybrene (Sigma-Aldrich, St. Louis, MO, USA). Two days after infection, single...
Xu et al. utilized anodic oxidation technology to create TiO2 nanoarrays on Ti plates. This allowed for gentle photothermal regulation of macrophage phenotype differentiation to M2 type, resulting in the regulation of bone regeneration. The study is particularly noteworthy for demonstrating the reversible...
Crystals of TRIM72 were grown in 24-well plates using the hanging drop vapor diffusion method by mixing 1.2 μL protein (15 mg/mL) with 1.2 μL crystallization buffer containing 100 mM HEPES pH 7.5, 11% (w/v) PEG 8000, 5% methanol. Crystal grew to full size after incubati...
ct comparison between symmetric and asymmetric baseplates eposter presentation thomas neri, md, phd, prof., france elise boulgakoff, ing, france christophe rascle, ing, france sven edward putnis, mb chb frcs(tr&orth), united kingdom antonio klasan, md, phd, emba, frcs, austria remi ...
10 when the cells reached 80% confluency. Only N2a cells with a passage number between one to fifteen were used for this study. For expression of GFP and GFP-tagged constructs in N2a cells, 18 mm glass coverslips (Thermo Scientific) were placed in 12-well plates (Costar) and were ...
Briefly, cells were seeded in 96 well plates. Serial dilutions of JQ1 and/or PARPi were added to the culture medium for 72 h. AlamarBlue reagent was applied according to the directions of the manufacturer, and fluorescence read on a Victor X5 microplate reader at 590 nm. 2.7. In ...