INDIANA ALTERS LICENSE PLATE RENEWAL SYSTEM
www.nature.com/scientificreports OPEN Self-renewal and phenotypic conversion are the main physiological responses of received: 28 July 2016 accepted: 07 February 2017 Published: 20 March 2017 macrophages to the endogenous estrogen surge Giovanna Pepe1, Daniele Braga2,3, Tiziana A. Renzi4,...
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Cells were plated at 5 × 103 per plate in the primary plating, followed by 2.5 × 104 per plate for secondary and tertiary plating, respectively. Surprisingly, the sizes of GATA2-ERT colonies in the absence of TAM were consistently larger than mock and control GFP vector colonies (Fig. 1...
We also differentiated AI-adapted hESCs into cardiomyocytes using the STEMdiff cardiomyocyte differentiation kit. qRT-PCR analysis confirmed upregulation ofGATA4,ISL1,TBX5,MEF2C,MLC2AandTNNT2lineage markers, and downregulation ofNANOG, OCT4andSOX2as differentiation progressed (Supplementary Fig.7a). Dif...
Homeobox genes are master regulators of cell fate during embryonic development and their expression is altered in cancer. By regulating the balance between cell proliferation and differentiation, they maintain homeostasis of normal tissues. Here, we scre
Using a zebrafish model of embryonal rhabdomyosarcoma (ERMS), we determined that Vangl2 expression enriches for TPCs and promotes their self-renewal. Expression of constitutively active and dominant-negative isoforms of RHOA revealed that it acts downstream of VANGL2 to regulate proliferation and ...
Cx26 forms a TNBC-specific complex with focal adhesion kinase (FAK) and NANOG. Cell lysates from bulk cell cultures ofamesenchymal-like TNBC (MDA-MB-231), basal-like TNBC (HCC70), TNBC (PDX TN-1), andbnon-TNBC (MCF7 and MCF10A) were subjected to immunoprecipitation with anti-Cx26,...
Our experimental results revealed that the caspase-8 medicated apoptotic signaling pathway is suppressed in hPSCs grown on the PET membrane as compared to those on a traditional tissue culture plate (TCP). These observations suggested the obstruction of cell self-renewal on a TCP surface. Treating ...