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(PTI, Monmouth Junction, NJ), equipped with a TLC50TM Legacy/PTI Peltier-based temperature-controlled cuvette chamber (Quantum Northwest). For photobleaching, photoisomerization and probe experiments the samples were irradiated with a current-controlled LED, either a Luxeon Rebel (peak wavelength ...
(BD FalconTM, NJ, USA). Single cell suspensions containing stem cells were plated onto Petri dishes (BD FalconTM) and cultured in NSC medium consisting of StemSure®DMEM (Wako, Tokyo, Japan), StemSure®Serum Replacement (SSR, 197-16775, Wako), MEM Non-essential amino acids, 0.1 ...
11 Cells were incubated with human recombinant GDF15 (PeproTech, Rocky Hill, NJ), the Akt-1/2 inhibitor (124018; EMD Millipore, Billerica, MA), or a mouse anti-human GDF15 monoclonal antibody (R&D Systems, Minneapolis, MN) for the indicated doses and time periods. For long-term ...