Two tied ligatures were placed around the celiac artery and mesenteric artery. One ligature was placed around inferior vena cava at the position right below right renal vein junction, into which heparin (100 units per mouse) was injected. After that, a ligature was placed at the proximal end...
we did not have the ability to alter this sex disparity. Only one study had both males and females, which enabled us to perform sex difference analyses for parameters such as blood and urine electrolytes