In the past two decades, various types of quantitative PCR (qPCR) approaches have been developed to quantify AMF (Janoušková and Caklová 2020). Approaches differ by the type of target primers, type of fluorescence technique, and type of data normalization. Quantitative PCR primers may targe...
4) phytoplankton abundance and growth in large volumes requires subsampling for measurement in a fluorometer, which exposes the experimental units to contamination, disturbance and greater effort, while experimental units that can be measured in situ in a fluorometer (e.g.,50 mL tubes) avoids these...
Fluorescence quantification was done by acquiring several images in different locations of each monolayer, and then measuring the integrated density (IntDen) of the appropriate channel using Fiji19. Quantitative polymerase chain reaction (qPCR) RNA was purified from cells using Direct-zol RNA miniprep ...
One liter of the filtrate containing the Halobacteriovorax cells (as determined by fluorescence microscopy) was centrifuged at 27,485 × g for 30 min. The pellet of predator cells was then resuspended in 6 ml of ASW. To test whether the concentrated Halobacteriovorax suspension was free of ...
distilled water. The reduction maintained SC linearity and reproducibility for normal (Supplementary Figure S2A) and aberrant (Supplementary Figure S2B)HBBmRNA, albeit at lower endpoint fluorescence (Supplementary Figure S2C), prompting our adoption of half-volume reactions for all subsequent RT-qPCR ...