ABI 3500 relative fluorescence unit (RFU) signal is approximately four times that of the ABI 3130 signal. As illustrated below, ABI 3500 noise levels may also be re-scaled giving rise to the visual appearance of noisier baselines. Generally speaking though, the signal-to-noise ratios are unc...
600 nM, 800 nM, 1000 nM) and four probe concentrations (50 nM, 100 nM, 150 nM, 200 nM) were tested. The optimal concentration for the two multiplex qPCRs (HPV16 or HPV18) was determined as the highest relative fluorescence unit (RFU) for the lowest Ct value (Table...