Cell growth regulation involves multiple growth factor receptors, where intracellular kinases activate or phosphorylate these receptors’ structural domains. This activation triggers downstream pro-growth signals through pathways like AKT (protein kinase B), protein kinase C (PKC), and MAP kinase, influen...
Plectin is a universal cytolinker protein that interlinks intermediate filaments and anchors them to the nuclear envelope and other organelles in the cytoplasm51, forming an interconnected network of biopolymers19. Therefore, depletion of plectin in plectin −/− fibroblasts causes aberrant changes ...
In addition, we speculated that the intracellular concentration of the regulator protein CatR may be another factor affecting the sensitivity and dynamic properties of the PMA promoter. Higher CatR intracellular concentration may provide more stringent regulation (less leakage), but lower sensitivity. To...
H, Casp-6high macrophages promoted IL-4-induced Arginase-1 activity of RAW264.7 cells compared with macrophages that transfected with control vector. I, Western blotting analysis showed that the protein levels of MMP-2 and MMP-9 were markedly increased in Casp-6high macrophages. Values were ...
However, our use of independent silencing tools and rescue experiments with target protein ectopic expression leaves no room for doubt. Imamura et al. demonstrated that while the effects of ZRF1 on cell proliferation were p53-dependent, those related to migration and invasion were p53-independent [...
The OsPP2C08-GFP fusion protein colocalized with the nuclear staining agent DAPI, indicating that OsPP2C08 is a nucleus-localized protein (Figure 1E). 2.2. Overexpression of OsPP2C08 in Rice Causes ABA Insensitive Phenotypes To study the function of OsPP2C08, we generated 15 transgenic ...
protein-protein interaction BAR domain CIP4 FBP17 TOCA1 WASP Introduction The Ras superfamily of small GTPases comprises over 150 members that regulate a multitude of cellular processes in eukaryotes. The superfamily can be divided into five families based on structural and functional similarities: Ras...
In top panel of LV-TGIF2-transduced cells, asterisks (*) indicate Albumin (ALB)-positive cells that are PDX1-negative; open arrowheads (4) indicate PDX1/GFP-positive cells that are ALB-negative in transduced cultures. In middle panels, arrows indicate examples of GFP-positive cells either ...
(b) Primary osteoblasts were cotransfected with the plasmids of green fluorescent protein (GFP)-NAA10 (2 mg) and Myc-Runx2 (1 mg) and treated with BMP-2 for 2 days. Protein expressions were analysed by western blotting. (c,d) C2C12 cells, which had been transfected with Runx2 siRNA ...