50. To identify regions of the genome with significant changes between the reference and query datasets, we selected regions with an ssim Z score less than −2 and a signal-to-noise ratio Z score of at least 1. For the box plots in Fig. 4c, windows were classified as containing DE ...
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Switchover stable cell lines were seeded in 6 well plates in the presence of 10 ng/ml doxycycline (Dox). The following day, the cells were transfected using Lipofectamine RNAiMax (Thermo Fisher Scientific) with a pool of two individual siRNAs (D-011186-03-0020 and D-011186-05-0020, Dharmac...
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The microbial DNA was extracted by the E.Z.N.A. Stool DNA kit (Omega inc., USA) and the total DNA was eluted in 50 μL of elution buffer, stored at -80℃ until measurement in the PCR (LC-Bio Technology Co., Ltd., China). The V3-V4 region of the prokaryotic 16S rRNA gene ...
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Forward peptides within three standard deviations of the theoretical m/z of the precursor were used as positive training set. All reverse peptides were used as negative training set. Linear Discriminant scores were used to sort peptides with at least seven residues and to filter with the ...
The other regulation process can operate at the protein level, as was demonstrated in a study of Z. mays, where TMP synthase activity was strongly inhibited by an increased supply of HMP-PP, produced through the activity of the kinase domain of the same protein, THI3 [11]. To test the ...
Nolan R. McGrady, Alena Z. Minton, Dorota L. Stankowska, Shaoqing He & Raghu R. Krishnamoorthy Texas College of Osteopathic Medicine, University of North Texas Health Science Center, 3500 Camp Bowie Blvd, Fort Worth, TX, 76107, USA Hayden B. Jefferies Corresponding author Correspondence to Rag...