However, except laforin, expression of these proteins is unchanged in malin knockout mice and thus malin's role in LD pathogenesis is still unclear. Glycogen phosphorylase and glycogen debranching enzyme are vital in catabolism of cellular glycogen. In this study, we aimed to discover substrate/s...
1983. Regulation of glycogen phosphorylase activity in fat body of Locusta migratoria and Periplaneta americana. Gen Comp Endocrinol 50: 226-234.Van Marrewijk WJ, Van Den Broek AT, Beenakkers AMT: Regulation of glycogen phospho- rylase activity in fat body of Locusta migratoria and Periplaneta ...
Uhing RJ, Janski AM, Graves DJ (1979) The effect of solvents on nucleotide regulation of glycogen phosphorylase. J Biol Chem 254:3166–3169Uhing RJ, Janski AM, Graves DJ. The effect of solvents on nucleotide regulation of glycogen phosphorylase. J Biol Chem. 1979 May 10; 254 (9):3166–...
On the other hand, glycogen degradation requires the glycogen debranching enzyme (GDE; GlgX), which directly catalyzes the hydrolysis of α-(1→6)-linked glucose residues and the glycogen phosphorylase (GP; GlgP) to generate glucose-1-phosphate, which feeds into primary metabolism7. An ...
1. Glycogen phosphorylase of locust fat-body was partially purified by differential centrifugation and dissociation from glycogen particles at two pH values. 2. Optimum activity was obtained at pH6.6-6.7. 3. The calculated apparent K(m) values for glycogen and glucose 1-phosphate were 0.08% and...
Phosphorylase a varied between 85.5 and 91.5% without significant differences between periods. However, the rate of glycogenolysis was twofold higher during the stimulation at 50 Hz than it was at 15 Hz (P less than 0.05) and was related to the ATP turnover rate (r = 0.992). These ...
We have examined the effect of glucose and caffeine inhibition on the activity of liver glycogen phosphorylase a from the freeze-tolerant frog Rana sylvatica. Kinetic studies indicate that this enzyme exhibits similar sensitivity to glucose inhibition (glucose dissociation constant = 12.5 mM) as the ...
The phosphatase activity, which was stimulated by MnCl, (1 to 10 mM) but not by MgCl,, catalyzed dephosphorylation of 3'P-glycogen synthase, 32P-phosphorylase, and 3'P-histones. By utilizing the differences in phosphorylation specificities displayed by the CAMP-dependent protein kinase catalytic ...
Phosphorylasea activity was assayed in the presence of caffeine (30). Twenty-five microliters of supernatant and 25 μl of assay buffer (100 mm glucose 1-phosphate, 2% glycogen, 300 mmsodium fluoride, 1 mm caffeine, pH 6.1) were combined and incubated for 45 min at 30 °C. Inorganic ...
Dephosphorylated glycogen phosphorylase is inert, whereas phosphorylated glycogen phosphorylase catalyzes the breakdown of glycogen. Epinephrine (Adrenaline) The adrenal glands release the hormone epinephrine, sometimes known as adrenaline, during times of stress or physical activity. To provide a quick supply...