The Swi5 protein bindsHO promoter DNA cooperatively with the Pho2 homeodomain protein, and the HO specificity region of Swi5 identified in the chimeric analysis coincides with the region of Swi5 previously identified that interacts with Pho2 in vitro. Swi5 and Ace2 also activate expression of ...
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Inverted terminal repeats (ITRs) of the adeno-associated virus (AAV) are essential for rescue, replication, packaging, and integration of the viral genome. While ITR mutations have been identified in previous reports, we designed a new truncated ITR lacking the B-B’ and C-C’ regions named ...
The functionality of genome regions is intrinsically related with their ability to fold in tri-dimensional structures, which clearly indicates that positional bias should be incorporated in the models and analyzed from a statistical point of view. A number of recent studies have focused on this prope...
Next-generation sequencing-based methods are extensively applied in studies of the human microbiota using partial 16 S rRNA gene amplicons. However, they carry drawbacks that are critical to consider when interpreting results, including differences in
Variation in gene expression underlies interindividual variability in relevant traits including immune response. However, the genetic variation responsible for these gene expression changes remains largely unknown. Among the non-coding variants that coul
Positional distribution of the core motifs CACCC (a), GATAAG (b), GATAAG (c), CACGTG (d) and CTTATC (e) in the promoter sequences of theAft2p,Dal80p,Gln3p,Met4pandGat1pdata sets, respectively. Full size image Dal80p This data set includes 26 promoter sequences of genes documente...
the 7th position had the highest fre-quency and guanine nucleotides at the 6th and the 7th positions were recognized in all uncleotide sequences.The latter sug-gests a crucial role of these guanines for the interatction of DNA with SF-1 protein.The determined consensus and frequen-cy ...
PCR was performed using the NEBNext High-Fidelity 2X PCR Master Mix (NEB) with primers against the DNMT1 transcript with the alternative start site (5′-GCATAAGCTCACCACAAAGGA-3′ and 5′-GGCCTCGAGGAAGTAGAAGC-3′) and positive control primers (against TYK2, 5′-GTACTGCTACGATCCGACCA-3...
Methylation and acetylation of position-specific lysine residues in the N-terminal tail of histones H3 and H4 play an important role in regulating chromatin structure and function. In the case of H3-Lys4, H3-Lys9, H3-Lys27, and H4-Lys20, the degree of methyla-tion was variable from the...