All experiments were conducted in accordance with the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals, with approval from the Institutional Animal Care and Use Committee at the University of California, Davis and the Canadian Council on Animal Care guidelines, with...
Gene accession numbers refer to genes or DNA sequences about which further information can be found in the databases at the National Center for Biotechnical Information (NCBI) at the National Library of Medicine. Elsevier authors wishing to enable other scientists to use the accession numbers cited...
After extensive washing, bands were detected by enhanced chemiluminescence reagent (Bio-Rad) and imaged using the ChemiDoc™ imaging system (Bio-Rad) or ImageQuant™ LAS 500 (GE healthcare). CRISPR excision CRISPR excision was performed using all-in-one CRISPR/Cas9 vector system71. Briefly, ...
8 Wadsworth Center, New York State Department of Health, Albany, New York 12208, USA. 9 Yale Center for RNA Science and Medicine, New Haven, Connecticut 06520, USA. Correspondence and requests for materials should be addressed to J.C. (email: j.cheng@yale.edu) or to J.L. (email: ...
AWS HealthImaging Store, analyze, and share medical images AWS HealthScribe Automatically generate clinical notes with AI AWS HealthLake Make sense of health data Amazon Personalize Build real-time recommendations into your applications Amazon Polly Turn text into life-like speech Amazon Rekogniti...
(SARS-CoV-2), which causes COVID-19, appeared in early December 2019 in Wuhan, a city of 11 million populations in China’s Hubei province [90,92]. According to the World Health Organization (WHO), more than 621 million individuals worldwide have been infected with SARS-CoV-2 during ...
Glioblastoma multiforme (GBM; World Health Organization [WHO] grade IV) is the most common and most malignant primary tumor of the brain. Excessive proliferation, diffuse infiltration into surrounding brain tissue and suppression of antitumor immune surveillance contribute to the malignant phenotype of gl...
1Department of Psychological and Brain Sciences, Gill Center for Biomolecular Research, Indiana UniversitySummary Deze procedure beschrijft de verzameling van discrete bevroren hersengebieden om hoogwaardige eiwitten en RNA te verkrijgen met behulp van goedkope en algemeen beschikbare tools. Abstract Na...
Imaging Center, Department of Clinical Pathology, Kuopio University Hospital, Kuopio, Finland Jaana M. Hartikainen, Veli-Matti Kosma & Arto Mannermaa Saw Swee Hock School of Public Health, National University of Singapore, Singapore, Singapore Mikael Hartman Department of Surgery, National University He...
For immunofluorescence imaging, cells with or without the electroporated proteins were recovered for 3~6 h on poly-D-lysine-coated coverslips in 24-well plates. Then, the cells were washed by pre-warmed PBS three times to remove extracellular un-delivered proteins, then fixed in 4% (w/v...