A study was conducted with Hy-Line Brown laying hens to examine the effects of reduced protein diet, deficiency of arginine (Arg), and addition of crystalline Arg, citrulline (Cit) and guanidinoacetic acid (GAA) as substitutes for Arg. Hen performance, egg quality, serum uric acid, liver an...
Protein Expression Levels of DAT, VMAT2, and TH in the DS and NAc Given the region-specific differences in amphetamine- induced dopamine efflux between adolescent and adult rats, we sought to investigate the molecular mechanisms responsible for these differences. We used western blot analysis to ...
decreased bacterial richness has been linked to high carbohydrate73and high-fat/low-protein concentrations74, as found in the lean and Western diets, respectively, the latter result only in old-
β-catenin transcriptional activity and protein levels were reduced in P19[shβ-cat] cells.Jacob WongVirja MehtaAnastassia VoronovaJosée CoutuTammy RyanMichael SheltonIlona S. Skerjanc
To examine the effect of pineapple consumption on oxidative stress and antioxidant status of the heart, the cardiac protein carbonyl (cPC) content, MDA level and total antioxidant capacities of the HCD model were determined. There was a significance reduction of cPC content in the HCD group (0....
Injection of adult mice with sonic Hh (Shh)-IgG fusion protein (a Hh agonist) causes a significant increase in fat mass and body weight without affecting food consumption and fluid intake [8]. In agreement with this observation, inactivation of Hh signaling by injection of anti-Hh antibodies ...
(12)Protocol for auxin-inducible depletion of the RNA-binding protein PTBP1 in mouse embryonic stem cellsSTAR ProtocolsDecember 1, 2023Yaroslav Kainov, Anna Zhuravskaya, Eugene V. Makeyev(Thermo Fisher Scientific, cat#11668019) and 100 μL of Opti-MEMI (Thermo Fisher Scientific, cat#3198507...
Protein concentrations were determined by Pierce BCA assay (Thermo Scientific) and samples were normalized to total protein content. Chromatin immunoprecipitationChromatin immnuprecipiation against c-Jun was performed using Magna ChIP HiSense Chromatin Immunoprecipitation Kit (cat. no. 17-10460, Sigma), ...
Immunoblotting of b-actin using a rabbit polyclonal antibody that binds to the C- terminal of b-actin (Sigma) was included in all western blot analysis to ensure comparable protein loading. The primary antibodies used include anti-huApoE (Santa Cruz Biotechn, Cat# 13521), anti-IRS1 (Cell ...
(S) at amino acid residue 6 in a wild type pennycress SHP1 protein (e.g., SEQ ID NO:18). The S at residue 6 can be substituted with any appropriate amino acid (e.g., asparagine (N)). For example, a modified SHP1 polypeptide can include a single S substituted for the S at ...