roGFPs, targeted to different cell compartments, are successfully used as specific markers of cell toxicity, induced by expression of causative genes linked to a determined ND. We also report the use of roGFP to monitor oxidative stress induced by the expression of the ALS-causative gene SOD1....
However, most of the roFPs suffer from either poor brightness or high pH-dependency, or both. Moreover, there is no roRFP with the redox potential close to that of 2GSH/GSSG redox pair. In the present work, we describe Grx1-roCherry, the first red roFP with canonical FP topology ...
Spot 1 is an (ataicm)idbeiycafpstieedpretH,ida2feOtes2retqHrue2aeOtnm2cienngt. with MS/MS. and recovered for 15 h to obtain the maximum degradation in fresh media containing CHX (25 μg/mL), and various concentrations of lactacystin, an irreversible proteasome inhibitor, or 3...