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The homogenized samples were then stored overnight at 4 ◦C after which it was centrifuged at 3220 g for 1 h at 4 ◦C (Eppendorf 5810R centrifuge, Brinkman Instruments Inc., Westbury, NY, USA) and then passed through Whatman # 1 filter paper to obtain 5% w/w spiked raw ground ...